| Title | High-Throughput Study of the Effects of Celastrol on Activated Fibroblast-Like Synoviocytes from Patients with Rheumatoid Arthritis |
| Authors | Fang, Zhengyu He, Dongyi Yu, Bo Liu, Feng Zuo, Jianping Li, Yuxia Lin, Qi Zhou, Xiaodong Wang, Qingwen |
| Affiliation | Shenzhen Peking Univ Hong Kong Univ Sci & Technol, Biomed Res Inst, Shenzhen 518036, Peoples R China. Peking Univ, Dept Rheumatism & Immunol, Shenzhen Hosp, Shenzhen 518036, Peoples R China. Guanghua Integrat Med Hosp, Shanghai 200052, Peoples R China. Peking Univ, Dept Dermatol, Shenzhen Hosp, Shenzhen 518036, Peoples R China. Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China. Univ Texas Hlth Sci Ctr Houston, Dept Internal Med, Houston, TX 77030 USA. Peking Univ, Dept Rheumatism & Immunol, Shenzhen Hosp, Shenzhen 518036, Peoples R China. Zhou, XD (reprint author), Univ Texas Hlth Sci Ctr Houston, Dept Internal Med, Houston, TX 77030 USA. |
| Keywords | celastrol rheumatoid arthritis fibroblast-like synoviocyte chemokine NF-B signaling pathway NF-KAPPA-B SYNOVIAL FIBROBLASTS EXPRESSION CELL PHOSPHORYLATION INFLAMMATION HYPERPLASIA SUPPRESSION THERAPY |
| Issue Date | 2017 |
| Publisher | GENES |
| Citation | GENES.2017,8(9). |
| Abstract | Celastrol, a natural triterpene, exhibits potential anti-inflammatory activity in a variety of inflammatory diseases. The present study aimed to investigate its biological effect on activated fibroblast-like synoviocytes (FLSs) from patients with rheumatoid arthritis (RA). The primary FLSs of the synovial tissues were obtained from synovial biopsies of patients with RA. The normal human FLS line (HFLS) was used as a control. After the RA-FLSs and HFLSs were treated with or without celastrol, various approaches, including the WST-1 assay, transwell assay, real-time PCR and ELISA analysis, were performed to estimate proliferation, invasion and expression of pro-inflammatory cytokines of the RA-FLSs. Microarray analysis was performed to screen for differentially expressed genes in RA-FLSs before and after celastrol treatment. The results showed that treatment of celastrol attenuated both the proliferation and invasion of the RA-FLSs. The expression of several chemokine genes, including CCL2, CXCL10, CXCL12, CCR2 and CXCR4, was significantly changed after celastrol treatment. The genes involved in the NF-B signaling pathway appeared to be regulated by celastrol. |
| URI | http://hdl.handle.net/20.500.11897/471002 |
| ISSN | 2073-4425 |
| DOI | 10.3390/genes8090221 |
| Indexed | SCI(E) |
| Appears in Collections: | 化学生物学与生物技术学院 深圳医院 |
