| Title | Epigenomic Landscape of Human Fetal Brain, Heart, and Liver |
| Authors | Yan, Liying Guo, Hongshan Hu, Boqiang Li, Rong Yong, Jun Zhao, Yangyu Zhi, Xu Fan, Xiaoying Guo, Fan Wang, Xiaoye Wang, Wei Wei, Yuan Wang, Yan Wen, Lu Qiao, Jie Tang, Fuchou |
| Affiliation | Peking Univ, Biodynam Opt Imaging Ctr, Beijing 100871, Peoples R China. Peking Univ, Ctr Reprod Med, Coll Life Sci, Dept Obstet & Gynecol,Hosp 3, Beijing 100871, Peoples R China. Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China. Minist Educ, Key Lab Assisted Reprod, Beijing 100191, Peoples R China. Minist Educ, Key Lab Cell Proliferat & Differentiat, Beijing 100871, Peoples R China. Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing 100871, Peoples R China. Peking Univ, Hlth Sci Ctr, Ctr Mol & Translat Med, Beijing 100191, Peoples R China. Peking Univ, Biodynam Opt Imaging Ctr, Beijing 100871, Peoples R China. Qiao, J Tang, FC (reprint author), Peking Univ, Ctr Reprod Med, Coll Life Sci, Dept Obstet & Gynecol,Hosp 3, Beijing 100871, Peoples R China. Qiao, J Tang, FC (reprint author), Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China. Qiao, J (reprint author), Minist Educ, Key Lab Assisted Reprod, Beijing 100191, Peoples R China. Tang, FC (reprint author), Minist Educ, Key Lab Cell Proliferat & Differentiat, Beijing 100871, Peoples R China. Qiao, J (reprint author), Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing 100871, Peoples R China. Tang, FC (reprint author), Peking Univ, Hlth Sci Ctr, Ctr Mol & Translat Med, Beijing 100191, Peoples R China. |
| Keywords | EMBRYONIC STEM-CELLS CHROMATIN STATE HUMAN GENOME HISTONE MODIFICATIONS SUPER-ENHANCERS TRANSCRIPTION FACTORS INTEGRATIVE ANALYSIS DNA ELEMENTS RNA-SEQ IN-VIVO |
| Issue Date | 2016 |
| Publisher | JOURNAL OF BIOLOGICAL CHEMISTRY |
| Citation | JOURNAL OF BIOLOGICAL CHEMISTRY.2016,291,(9),4386-4398. |
| Abstract | The epigenetic regulation of spatiotemporal gene expression is crucial for human development. Here, we present whole-genome chromatin immunoprecipitation followed by high throughput DNA sequencing (ChIP-seq) analyses of a wide variety of histone markers in the brain, heart, and liver of early human embryos shortly after their formation. We identified 40,181 active enhancers, with a large portion showing tissue-specific and developmental stage-specific patterns, pointing to their roles in controlling the ordered spatiotemporal expression of the developmental genes in early human embryos. Moreover, using sequential ChIP-seq, we showed that all three organs have hundreds to thousands of bivalent domains that are marked by both H3K4me3 and H3K27me3, probably to keep the progenitor cells in these organs ready for immediate differentiation into diverse cell types during subsequent developmental processes. Our work illustrates the potentially critical roles of tissue-specific and developmental stage-specific epigenomes in regulating the spatiotemporal expression of developmental genes during early human embryonic development. |
| URI | http://hdl.handle.net/20.500.11897/437653 |
| ISSN | 0021-9258 |
| DOI | 10.1074/jbc.M115.672931 |
| Indexed | SCI(E) EI |
| Appears in Collections: | 生物医学前沿创新中心 第三医院 生命科学学院 |
