Title | Clinical Performance of APTIMA Human Papillomavirus (HPV) 16 18/45 mRNA Genotyping Testing for the Detection of Cervical Intraepithelial Neoplasia 3 (CIN3) or Cancer in a Select Group of Chinese Women |
Authors | Guo, Yan-li You, Ke Geng, Li Qiao, Jie |
Affiliation | Peking Univ, Hosp 3, Dept Obstet & Gynecol, 49 Huayuanbei Rd, Beijing 100191, Peoples R China. |
Keywords | Human papillomavirus HPV mRNA genotyping Cervical cancer Screening HYBRID CAPTURE-2 VIRAL LOAD TRANSIENT INFECTIONS SCREENING-TEST RISK CYTOLOGY PREVENTION GUIDELINES AMERICAN SOCIETY |
Issue Date | 2016 |
Publisher | PATHOLOGY & ONCOLOGY RESEARCH |
Citation | PATHOLOGY & ONCOLOGY RESEARCH.2016,22(3),549-554. |
Abstract | HPV type was evaluated in a select group of Chinese women that were positive with hybrid capture, and correlations were performed between the pathology found, the type of virus and a semiquantitation from the hybrid capture results. Totally 394 referred high-risk-HPV-positive women evaluated by Hybrid Capture 2 (HC-2) assay were enrolled. Before colposcopy, cervical specimens were collected from all participants and suspended into PreservCytcollection medium (Hologic Inc., Marlborough, MA), and tested with the APTIMA HPV16 18/45 mRNA assay. Colposcopy and diagnostic biopsies were done on all participants. Viral load was assessed by HC2 assay. Totally 55 women were diagnosed as CIN 3 plus cancer (a parts per thousand yenCIN3), and the prevalence of HPV16/18/45 was 65.5 % (95 % confidence interval [CI], 52.9-78.0 %) among these a parts per thousand yenCIN3 women. Compared with the group with positive HC2 but negative HPV16/18/45, the odds ratio (OR) to identify a parts per thousand yenCIN3 was 6.3 (95 % CI, 3.2-12.3) for HPV16 and 3.2 (95 % CI, 1.4-7.2) for HPV18/45. When using a parts per thousand yenCIN3 as an endpoint, the sensitivity and specificity was 65.5 % (95 % CI, 52.9-78.0 %) and 72.0 % (95 % CI, 67.2-76.8 %). In the case of HPV16/18/45 negative, no high HPV load had a statistically significant increased risk for the prevalence of a parts per thousand yenCIN3. HPV16, 18 and 45 infection is a major cause for a parts per thousand yenCIN3 in Chinese women. Women with positive HPV16/18/45 should be referred to colposcopy immediately. HPV load was not suitable for the further triaged of the HPV16/18/45 negative cases. |
URI | http://hdl.handle.net/20.500.11897/435625 |
ISSN | 1219-4956 |
DOI | 10.1007/s12253-015-0029-2 |
Indexed | SCI(E) PubMed |
Appears in Collections: | 第三医院 |