| Title | The Dorsal Raphe Nucleus Receives Afferents From Alpha-Like Retinal Ganglion Cells and Intrinsically Photosensitive Retinal Ganglion Cells in the Rat |
| Authors | Li, Xiaotao Ren, Chaoran Huang, Lu Lin, Bin Pu, Mingliang Pickard, Gary E. So, Kwok-Fai |
| Affiliation | Univ Hong Kong, Li Ka Shing Fac Med, Sch Biomed Sci, Pokfulam, Hong Kong, Peoples R China. Univ Hong Kong, Li Ka Shing Fac Med, Dept Ophthalmol, Pokfulam, Hong Kong, Peoples R China. Univ Hong Kong, State Key Lab Brain & Cognit Sci, Pokfulam, Hong Kong, Peoples R China. Univ Hong Kong, Li Ka Shing Fac Med, Res Ctr Heart Brain Hormone & Hlth Aging, Pokfulam, Hong Kong, Peoples R China. Chinese Acad Sci, Shenzhen Inst Adv Technol, Brain Cognit & Brain Dis Inst Collaborat Res, Shenzhen, Peoples R China. Chinese Acad Sci, Shenzhen Inst Adv Technol, McGovern Inst MIT, Shenzhen, Peoples R China. Jinan Univ, GHM Inst CNS Regenerat, Guangzhou, Guangdong, Peoples R China. Peking Univ, Dept Anat, Sch Basic Med Sci, Beijing 100871, Peoples R China. Peking Univ, Minist Educ, Key Lab Machine Percept, Beijing 100871, Peoples R China. Peking Univ, Minist Educ, Key Lab Visual Impairment & Restorat, Beijing 100871, Peoples R China. Univ Nebraska, Sch Vet Med & Biomed Sci, Lincoln, NE USA. Univ Nebraska Med Ctr, Dept Ophthalmol & Visual Sci, Omaha, NE USA. Univ Hong Kong, Li Ka Shing Fac Med, L1-55,21 Sassoon Rd, Pokfulam, Hong Kong, Peoples R China. |
| Keywords | dorsal raphe nucleus retinal ganglion cell melanopsin ipRGCs retinofugal projection C-FOS EXPRESSION SEROTONERGIC NEURONS CENTRAL PROJECTIONS MIDBRAIN RAPHE MELANOPSIN LIGHT DEGENERATION RETROGRADE DEPRESSION ANXIETY |
| Issue Date | 2015 |
| Publisher | INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE |
| Citation | INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE.2015,56,(13),8373-8381. |
| Abstract | PURPOSE. A retinal projection into the dorsal raphe nucleus (DRN), namely, the retino-raphe projection, exists in many species. The rat is one of several species in which a retino-raphe projection has been described; however, the retinal ganglion cell (RGC) types that contribute to this pathway are unknown. METHODS. Retrograde tracing via cholera toxin subunit B (CTB) was used to reveal DRN-projecting RGCs in rats, combined with intracellular injection in vitro, melanopsin immunostaining in whole-mounted retinas, and serotonin immunostaining to define the DRN. We modified methods of CTB injection into DRN used previously in order to avoid possible contamination with other retinorecipient regions, particularly the superior colliculus (SC). RESULTS. The majority of DRN-projecting RGCs showed alpha-like morphology, and some CTB-positive RGCs were colabeled with melanopsin. Approximately 80% of the total population of CTB-labeled DRN-projecting RGCs was alpha-like cells including ON alpha cells and OFF alpha cells; these alpha-like cells were melanopsin immunonegative. Approximately 10% of the remaining DRN-projecting RGCs were melanopsin immunopositive, in which the M1 subtype of intrinsically photosensitive retinal ganglion cell (ipRGC) provided the dominant projection of ipRGCs into DRN, with only few non-M1 ipRGCs involved. The DRN-projecting ipRGCs could be retrogradely labeled following tracer injection into all rostrocaudal aspects of the DRN. CONCLUSIONS. Both conventional RGCs with alpha-like morphology and melanopsin-expressing ipRGCs project into the rat DRN. Approximately 10% of DRN-projecting RGCs were colabeled with melanopsin, and the majority of these were the M1 subtype of ipRGCs. An ipRGC component of the retino-raphe projection may contribute to a sustained light-mediated modulation of DRN serotonin release. |
| URI | http://hdl.handle.net/20.500.11897/435446 |
| ISSN | 0146-0404 |
| DOI | 10.1167/iovs.15-16614 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 基础医学院 机器感知与智能教育部重点实验室 |
