| Title | Label-free quantitative proteomic analysis of benzo(a)pyrene-transformed 16HBE cells serum-free culture supernatant and xenografted nude mice sera |
| Authors | Zhao, Peng Fu, Juanling Yao, Biyun Jia, Yongrui Zhang, Hongtao Li, Xuehui Dong, Lisha Gao, Ya Liu, Wenli Chen, Wen Zhou, Zongcan |
| Affiliation | Peking Univ, Dept Toxicol, Hlth Sci Ctr, Beijing 100191, Peoples R China. Peking Univ, Hlth Sci Ctr, Beijing Key Lab Toxicol Res & Risk Assessment Foo, Beijing 100191, Peoples R China. Peking Univ, Med & Hlth Analyt Ctr, Hlth Sci Ctr, Beijing 100191, Peoples R China. Sun Yat Sen Univ, Sch Publ Hlth, Fac Prevent Med, Dept Toxicol, Guangzhou 510080, Guangdong, Peoples R China. |
| Keywords | Label-free quantitative proteomics NRP2 CLU AKAP12 Benzo(a)pyrene T-16HBE-C1 cells POLYCYCLIC AROMATIC-HYDROCARBONS BRONCHIAL EPITHELIAL-CELLS LYMPH-NODE METASTASIS LUNG-CANCER CELLS MALIGNANT-TRANSFORMATION NEUROPILIN-2 EXPRESSION POOR-PROGNOSIS OVARIAN-CANCER CLUSTERIN OSTEOSARCOMA |
| Issue Date | 2016 |
| Publisher | CHEMICO-BIOLOGICAL INTERACTIONS |
| Citation | CHEMICO-BIOLOGICAL INTERACTIONS.2016,245,39-49. |
| Abstract | To screen potential biomarkers of benzo(a) pyrene (BaP)-induced lung cancer, the proteomic profiles of BaP-transformed 16HBE cell line T-16HBE-C1 cells serum-free culture supernatant and xenografted nude mice sera were compared with those of 16HBE group by utilizing label-free quantitative proteomic strategy. By employing nano-LC-MS/MS technology followed by MaxQuant and Perseus processing, 489 differentially expressed proteins were identified between T-16HBE-C1 and 16HBE cells serum-free culture supernatant, and 49 significantly up-regulated proteins were identified in T-16HBE-C1 xenografted nude mice sera. Three proteins neuropilin-2 (NRP2), clusterin (CLU) and A-kinase anchor protein 12 (AKAP12) were up-regulated in the serum-free culture supernatant of T-16HBE-C1 cells. These 3 human proteins were present in the sera of nude mice xenografted with T-16HBE-C1 cells, but were undetectable in mice xenografted with 16HBE cells. The proteomic results of NRP2 and AKAP12 were confirmed by Western blotting and enzyme-linked immunosorbent assays, respectively. Moreover, the serum NRP2 levels were significantly elevated at the 4th day after tumor cell implantation and showed good positive correlation with tumor growth characterized by tumor volume. In conclusion, serum NRP2, CLU and AKAP12 could be potential biomarkers of BaP-induced lung cancer. The proteomic results will gain deeper insights into the mechanisms of BaP-induced carcinogenesis. (C) 2016 Elsevier Ireland Ltd. All rights reserved. |
| URI | http://hdl.handle.net/20.500.11897/435444 |
| ISSN | 0009-2797 |
| DOI | 10.1016/j.cbi.2015.12.017 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 医学部待认领 |
