| Title | Neuroprotective Effects of A Standardized Flavonoid Extract of Safflower Against Neurotoxin-Induced Cellular and Animal Models of Parkinson's Disease |
| Authors | Ren, Rutong Shi, Chunyan Cao, Jing Sun, Yi Zhao, Xin Guo, Yongfei Wang, Chen Lei, Hui Jiang, Hanjie Ablat, Nuramatjan Xu, Jiamin Li, Wan Ma, Yingcong Qi, Xianrong Ye, Min Pu, Xiaoping Han, Hongbin |
| Affiliation | Peking Univ, Sch Pharmaceut Sci, Dept Mol & Cellular Pharmacol, Beijing 100191, Peoples R China. Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China. Beijing Key Lab MRI Device & Tech, Beijing 100191, Peoples R China. Peking Univ, Hosp 3, Dept Radiol, Beijing 100191, Peoples R China. Peking Univ, Sch Pharmaceut Sci, Dept Mol & Cellular Pharmacol, Beijing 100191, Peoples R China. Pu, XP (reprint author), Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China. Han, HB (reprint author), Beijing Key Lab MRI Device & Tech, Beijing 100191, Peoples R China. Han, HB (reprint author), Peking Univ, Hosp 3, Dept Radiol, Beijing 100191, Peoples R China. |
| Keywords | BRAIN EXTRACELLULAR-SPACE PROTECTS DOPAMINERGIC-NEURONS CARTHAMUS-TINCTORIUS ALPHA-SYNUCLEIN OXIDATIVE STRESS DIFFUSION RATS STRIATUM VOLUME NMDA |
| Issue Date | 2016 |
| Publisher | SCIENTIFIC REPORTS |
| Citation | SCIENTIFIC REPORTS.2016,6. |
| Abstract | Safflower has long been used to treat cerebrovascular diseases in China. We previously reported that kaempferol derivatives of safflower can bind DJ-1, a protein associated with Parkinson's disease (PD), and flavonoid extract of safflower exhibited neuroprotective effects in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine- induced mouse model of PD. In this study, a standardized safflower flavonoid extract (SAFE) was isolated from safflower and mainly contained flavonoids. Two marker compounds of SAFE, kaempferol 3-O-rutinoside and anhydrosafflor yellow B, were proven to suppress microtubule destabilization and decreased cell area, respectively. We confirmed that SAFE in dripping pill form could improve behavioural performances in a 6-hydroxydopamine (6-OHDA)-induced rat model of PD, partially via the suppression of a-synuclein overexpression or aggregation, as well as the suppression of reactive astrogliosis. Using an MRI tracer-based method, we found that 6-OHDA could change extracellular space (ECS) diffusion parameters, including a decrease in tortuosity and the rate constant of clearance and an increase in the elimination half-life of the tracer in the 6-OHDA-lesioned substantia nigra. SAFE treatment could partially inhibit the changes in ECS diffusion parameters, which might provide some information about neuronal loss and astrocyte activation. Consequently, our results indicate that SAFE is a potential therapeutic herbal product for treatment of PD. |
| URI | http://hdl.handle.net/20.500.11897/434775 |
| ISSN | 2045-2322 |
| DOI | 10.1038/srep22135 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 药学院 第三医院 天然药物与仿生药物国家重点实验室 |
