| Title | Molecular characterization and antibiotic resistance of clinical Streptococcus dysgalactiae subsp equisimilis in Beijing, China |
| Authors | Lu, Binghuai Fang, Yujie Huang, Lei Diao, Baowei Du, Xiaoli Kan, Biao Cui, Yanchao Zhu, Fengxia Li, Dong Wang, Duochun |
| Affiliation | Civil Aviat Gen Hosp, Dept Lab Med, Beijing 100123, Peoples R China. Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing 102206, Peoples R China. Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou 310003, Zhejiang, Peoples R China. Peking Univ, Hosp 1, Dept Lab Med, Beijing 100034, Peoples R China. Civil Aviat Gen Hosp, Dept Lab Med, Beijing 100123, Peoples R China. Wang, DC (reprint author), Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing 102206, Peoples R China. |
| Keywords | Molecular characterization Antibiotic resistance S. dysgalactiae subsp equisimilis China BETA-HEMOLYTIC STREPTOCOCCI GROUP-A ANTIGEN INVASIVE INFECTIONS PREGNANT-WOMEN SURVEILLANCE PYOGENES GENES JAPAN EMM |
| Issue Date | 2016 |
| Publisher | INFECTION GENETICS AND EVOLUTION |
| Citation | INFECTION GENETICS AND EVOLUTION.2016,40,119-125. |
| Abstract | Streptococcus dysgalactiae subsp. equisimilis (SDSE) is presently considered as a human pathogen associated with clinical infection. We characterized 56 SDSE isolates collected from two tertiary hospitals in Beijing, China. Sixteen distinct emm types/subtypes were detected, dominated by stG245.0 (32.1%), stG652.0 (10.7%), stG6.1 (10.7%) and stG485.0 (10.7%), and a novel stG840.0 variant type was identified. All isolates possessed virulence genes of sagA and scpA, and most carried slo (98.2%), ska (98.2%) and speG(dys) (35.7%). Bymultilocus sequence typing (MLST) analysis, 17 individual sequence types (STs) were distinguished, including 7 newly-identified STs (26.8% of isolates), of which ST127 (30.4%), ST7 (12.5%) and ST44 (10.7%) dominated. Meanwhile, pulsed-field gel electrophoresis (PFGE) analysis revealed 33 pattern types (PTs), which were further combined into 16 pattern clusters (PCs), and 59.3% of isolates were distributed into 2 dominant PCs. Notably, emm types had both close relationship and consistency with STs and PFGE PCs. Furthermore, of 56 SDSE isolates, the predominant antibiotic resistances were erythromycin (71.4%), clindamycin (71.4%) and tetracycline (60.7%). Correspondingly, the prevalent resistance genes of macrolide and tetracycline were erm(B) (78.6%) and tet(M) (73.2%). In addition, multiple point mutations of parC, one of fluoroquinolone resistance genes, were observed (accounting for 75%), and were divided into 12 types, with parC 07 as the predominant type. Our data suggested the wide molecular diversity and distinctive regional features of SDSE from clinical infection in Beijing, China. (c) 2016 Elsevier B.V. All rights reserved. |
| URI | http://hdl.handle.net/20.500.11897/434706 |
| ISSN | 1567-1348 |
| DOI | 10.1016/j.meegid.2016.01.030 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 第一医院 |
