| Title | Biological effects of agglomerated multi-walled carbon nanotubes |
| Authors | Song, Zheng-Mei Wang, Lin Chen, Ni Cao, Aoneng Liu, Yuanfang Wang, Haifang |
| Affiliation | Shanghai Univ, Inst Nanochem & Nanobiol, Shanghai 200444, Peoples R China. Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China. |
| Keywords | Multi-walled carbon nanotube Agglomeration Calcium ion Uptake Toxicity METAL-IONS TITANIUM-DIOXIDE CELLULAR UPTAKE GRAPHENE OXIDE IN-VITRO NANOPARTICLES TOXICITY CELLS CYTOTOXICITY ADSORPTION |
| Issue Date | 2016 |
| Publisher | COLLOIDS AND SURFACES B-BIOINTERFACES |
| Citation | COLLOIDS AND SURFACES B-BIOINTERFACES.2016,142,65-73. |
| Abstract | The physicochemical properties of nanomaterials play crucial roles in determining their biological effects. Agglomeration of nanomaterials in various systems is a common phenomenon, however, how agglomeration affects the biological consequence of nanomaterials has not been well investigated because of its complexity. Herein, we prepared variable sized agglomerates of oxidized multi-walled carbon nanotubes (O-MWCNTs) by using Ca2+ and studied their cellular uptake and cytotoxicity in HeLa cells. We found the altered property of O-MWCNTs agglomerates could be controlled and adjusted by the amount of Ca2+. Agglomeration remarkably facilitated the cellular uptake of O-MWCNTs at the initial contact stage, due to the easy contact of agglomerates with cells. But agglomeration did not induce evident cytotoxicity when the concentration of O-MWCNTs was less than 150 mu g/mL. That was assayed by cell proliferation, membrane integrity, apoptosis and ROS generation. This study suggests us that the biological behaviors of nanomaterials could be altered by their states of agglomeration. (C) 2016 Elsevier B.V. All rights reserved. |
| URI | http://hdl.handle.net/20.500.11897/434682 |
| ISSN | 0927-7765 |
| DOI | 10.1016/j.colsurfb.2016.02.032 |
| Indexed | SCI(E) EI PubMed |
| Appears in Collections: | å å¦ä¸ å å å·¥ç¨ å¦é ¢ |
