| Title | Sparse C-13 labelling for solid-state NMR studies of P. pastoris expressed eukaryotic seven-transmembrane proteins |
| Authors | Liu, Jing Liu, Chang Fan, Ying Munro, Rachel A. Ladizhansky, Vladimir Brown, Leonid S. Wang, Shenlin |
| Affiliation | Peking Univ, Beijing NMR Ctr, Beijing 100871, Peoples R China. Peking Univ, Coll Chem & Mol Engn, Beijing 100871, Peoples R China. Beijing Natl Lab Mol Sci, Beijing, Peoples R China. Scripps Res Inst, La Jolla, CA 92037 USA. Univ Guelph, Dept Phys, Guelph, ON N1G 2W1, Canada. Univ Guelph, Biophys Interdept Grp, Guelph, ON N1G 2W1, Canada. Peking Univ, Beijing NMR Ctr, Beijing 100871, Peoples R China. Wang, SL (reprint author), Peking Univ, Coll Chem & Mol Engn, Beijing 100871, Peoples R China. Wang, SL (reprint author), Beijing Natl Lab Mol Sci, Beijing, Peoples R China. |
| Keywords | Magic-angle-spinning solid-state NMR Sparse C-13 isotope labelling Eukaryotic seven-transmembrane proteins P. pastoris expression system ANABAENA SENSORY RHODOPSIN M2 PROTON CHANNELS MEMBRANE-PROTEIN PICHIA-PASTORIS AMYLOID FIBRILS SPECTROSCOPY ASSIGNMENT RESOLUTION DILUTION BILAYERS |
| Issue Date | 2016 |
| Publisher | JOURNAL OF BIOMOLECULAR NMR |
| Citation | JOURNAL OF BIOMOLECULAR NMR.2016,65(1),7-13. |
| Abstract | We demonstrate a novel sparse C-13 labelling approach for methylotrophic yeast P. pastoris expression system, towards solid-state NMR studies of eukaryotic membrane proteins. The labelling scheme was achieved by co-utilizing natural abundance methanol and specifically C-13 labelled glycerol as carbon sources in the expression medium. This strategy improves the spectral resolution by 1.5 fold, displays site-specific labelling patterns, and has advantages for collecting long-range distance restraints for structure determination of large eukaryotic membrane proteins by solid-state NMR. |
| URI | http://hdl.handle.net/20.500.11897/433938 |
| ISSN | 0925-2738 |
| DOI | 10.1007/s10858-016-0033-1 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 化学与分子工程学院 |
