| Title | Nanoprobes for two-photon excitation time-resolved imaging of living animals: In situ analysis of tumor-targeting dynamics of nanocarriers |
| Authors | Yang, Wen Fu, Li-Min Wen, Xue Liu, Ying Tian, Ye Liu, Yu-Chen Han, Rong-Cheng Gao, Zhi-Yue Wang, Tian-En Sha, Yin-Lin Jiang, Yu-Qiang Wang, Yuan Zhang, Jian-Ping |
| Affiliation | Peking Univ, Coll Chem & Mol Engn, State Key Lab Struct Chem Unstable & Stable Speci, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China. Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China. Renmin Univ China, Dept Chem, Beijing 100872, Peoples R China. Peking Univ, Sch Basic Med Sci, Dept Biophys, Beijing 100191, Peoples R China. Peking Univ, Biomed X Ctr, Beijing 100191, Peoples R China. Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Mol Dev Biol, Beijing 100101, Peoples R China. Peking Univ, Coll Chem & Mol Engn, State Key Lab Struct Chem Unstable & Stable Speci, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China. Wang, Y (reprint author), Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China. Zhang, JP (reprint author), Renmin Univ China, Dept Chem, Beijing 100872, Peoples R China. |
| Keywords | Nanoprobes Nanocarriers Tumor-targeting dynamics Two-photon-excitation Time-resolved imaging Noninvasive luminescence analysis CANCER-THERAPY EUROPIUM COMPLEX DRUG-DELIVERY QUANTUM DOTS NANOPARTICLES LUMINESCENCE TRANSFERRIN MICROSCOPY NM ACCUMULATION |
| Issue Date | 2016 |
| Publisher | BIOMATERIALS |
| Citation | BIOMATERIALS.2016,100,152-161. |
| Abstract | Great challenges remain in the noninvasive luminescence imaging analysis of tumor-targeting dynamics of nanocarriers in living animals which is of significance for the development of anti-cancer nano medicine. In this work, luminescent nanoparticles Eu(tta)(3)bpt@SMA (d(av), = 15 nm), which exhibited good water dispersion stability and high yields of red Eu-luminescence under near-infrared two-photon excitation, were prepared by a modified microfluidic mixing method in the absence of surfactants. Tumor-targeting agents, Arg-Gly-Asp-D-Phe-Lys (cRGD) polypeptide or transferrin (Tf), were then anchored on the nanoparticle surfaces to form the desired nanocarriers Eu@SMA-RGD or Eu@SMA-Tf. The tumor-targeting processes of the prepared nanocarriers in intact living mice were analyzed on a home-built two-photon excitation time-resolved (TPE-TR) imaging apparatus having a wide view filed. The TPE-TR strategy could effectively suppress the interference from biological autofluorescence, which allowed the targeted domains to be visualized with a high signal-to-noise ratio. It was found that the tumor-tissue trapping efficacy of Eu@SMA-RGD was much higher than that of Eu@SMA-Tf, and the desorption process from the tumor tissues of Eu@SMA-RGD was slower than that of Eu@SMA-Tf. The methods developed in this work pave a way to investigate the in vivo tumor-targeting dynamics of nanocarriers by noninvasive luminescence imaging of living animals. (C) 2016 Elsevier Ltd. All rights reserved. |
| URI | http://hdl.handle.net/20.500.11897/433480 |
| ISSN | 0142-9612 |
| DOI | 10.1016/j.biomaterials.2016.05.032 |
| Indexed | SCI(E) EI PubMed |
| Appears in Collections: | å å¦ä¸ å å å·¥ç¨ å¦é ¢ 前沿交叉学科研究院 基础医学院 |
