| Title | Application of Isothermal Amplification Techniques for Identification of Madurella mycetomatis, the Prevalent Agent of Human Mycetoma |
| Authors | Ahmed, Sarah A. van de Sande, Wendy W. J. Desnos-Ollivier, Marie Fahal, Ahmed H. Mhmoud, Najwa A. de Hoog, G. S. |
| Affiliation | Univ Khartoum, Fac Med Lab Sci, Khartoum, Sudan. CBS KNAW Fungal Biodivers Ctr, Utrecht, Netherlands. Univ Amsterdam, Inst Biodivers & Ecosyst Dynam, NL-1012 WX Amsterdam, Netherlands. Erasmus MC, Dept Med Microbiol & Infect Dis, Rotterdam, Netherlands. Inst Pasteur, Unite Mycol Mol, Ctr Natl Reference Mycol & Antifong, Paris, France. Univ Khartoum, Mycetoma Res Ctr, Khartoum, Sudan. Peking Univ, Hlth Sci Ctr, Res Ctr Med Mycol, Beijing 100871, Peoples R China. Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Guangzhou 510275, Guangdong, Peoples R China. Second Mil Med Univ, Shanghai Inst Med Mycol, Changzheng Hosp, Shanghai, Peoples R China. Univ Fed Parana, Basic Pathol Dept, Curitiba, Parana, Brazil. King Abdulaziz Univ, Jeddah 21413, Saudi Arabia. |
| Keywords | RECOMBINASE POLYMERASE AMPLIFICATION BLACK-GRAIN EUMYCETOMA RAPID IDENTIFICATION CAUSATIVE AGENTS DNA DIAGNOSIS BURDEN FUNGI LAMP |
| Issue Date | 2015 |
| Publisher | JOURNAL OF CLINICAL MICROBIOLOGY |
| Citation | JOURNAL OF CLINICAL MICROBIOLOGY.2015,53,(10),3280-3285. |
| Abstract | Appropriate diagnosis and treatment of eumycetoma may vary significantly depending on the causative agent. To date, the most common fungus causing mycetoma worldwide is Madurella mycetomatis. This species fails to express any recognizable morphological characteristics, and reliable identification can therefore only be achieved with the application of molecular techniques. Recombinase polymerase amplification (RPA) and loop-mediated isothermal amplification (LAMP) are proposed as alternatives to phenotypic methods. Species-specific primers were developed to target the ribosomal DNA (rDNA) internal transcribed spacer (ITS) region of M. mycetomatis. Both isothermal amplification techniques showed high specificity and sufficient sensitivity to amplify fungal DNA and proved to be appropriate for detection of M. mycetomatis. Diagnostic performance of the techniques was assessed in comparison to conventional PCR using biopsy specimens from eumycetoma patients. RPA is reliable and easy to operate and has the potential to be implemented in areas where mycetoma is endemic. The techniques may be expanded to detect fungal DNA from environmental samples. |
| URI | http://hdl.handle.net/20.500.11897/418497 |
| ISSN | 0095-1137 |
| DOI | 10.1128/JCM.01544-15 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 医学部待认领 |
