| Title | SILAC-based quantitative proteomics identified lysosome as a fast response target to PDT agent Gd-N induced oxidative stress in human ovarian cancer IGROV1 cells |
| Authors | Qi, Dandan Wang, Qianqian Li, Hongguang Zhang, Tao Lan, Rongfeng Kwong, Daniel W. J. Wong, Wai-Kwok Wong, Ka-Leung Li, Shuiming Lu, Fei |
| Affiliation | Peking Univ, Shenzhen Grad Sch, Sch Chem Biol & Biotechnol, Lab Chem Genom, Shenzhen 518055, Peoples R China. Hong Kong Baptist Univ, Inst Res & Continuing Educ, Shenzhen 518057, Peoples R China. Hong Kong Baptist Univ, Dept Chem, Kowloon Tong, Hong Kong, Peoples R China. Shenzhen Univ, Coll Life Sci, Key Lab Marine Bioresource & Ecol, Shenzhen 518060, Peoples R China. S China Normal Univ, Minist Educ, Key Lab Laser Life Sci, Guangzhou 510631, Guangdong, Peoples R China. S China Normal Univ, Coll Biophoton, Inst Laser Life Sci, Guangzhou 510631, Guangdong, Peoples R China. |
| Keywords | PHOTODYNAMIC THERAPY PROTEIN BIOMARKERS CARCINOMA TISSUE |
| Issue Date | 2015 |
| Publisher | MOLECULAR BIOSYSTEMS |
| Citation | MOLECULAR BIOSYSTEMS.2015,11,(11),3059-3067. |
| Abstract | Biological systems have developed an intact network and strategies in response to various environmental pressures such as irradiation, viral invasion and oxidative stress. Therefore, elucidation of the cellular response mechanism toward oxidative stress can contribute to the knowledge of redox regulation. By using a newly developed gadolinium based photodynamic therapy (PDT) agent Gd-N and SILAC quantified proteomic analysis, we observed 485 proteins dysregulated in expression, 106 in phosphorylation and 1050 in oxidation. Interestingly, lysosome was discovered as the main organelle affected by Gd-N induced singlet oxygen, along with the down regulation of a majority of lysosomal acid hydrolases and proton pump complex ATP6V/TCIRG1. Besides, phosphorylation sites with sequence patterns "TP'' or "SP'' were enriched in dysregulated phosphoproteins. Protein oxidation also shows sequence patterns in target proteins with "M.D'' or "KM'' taking methionine as the central residue. Oxidized proteins were most enriched in the pathways of Parkinson's disease, an oxidative stress closely related neurodegenerative disease. In conclusion, our study reveals new insights into the cellular mechanism to oxidative stress and may contribute to the discovery of new targets and development of novel PDT agents. |
| URI | http://hdl.handle.net/20.500.11897/418233 |
| ISSN | 1742-206X |
| DOI | 10.1039/c5mb00497g |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 化学生物学与生物技术学院 |
