| Title | Lipidomic analysis of p-chlorophenylalanine-treated mice using continuous-flow two-dimensional liquid chromatography/quadrupole time-of-flight mass spectrometry |
| Authors | Weng, Rui Shen, Sensen Yang, Li Li, Min Tian, Yonglu Bai, Yu Liu, Huwei |
| Affiliation | Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn,Minist Educ, Inst Analyt Chem,Coll Chem & Mol Engn, Beijing 100871, Peoples R China. Peking Univ, Lab Anim Ctr, Beijing 100871, Peoples R China. Peking Univ, Coll Chem & Mol Engn, Inst Analyt Chem, Beijing 100871, Peoples R China. |
| Keywords | LC-QTOF-MS ALZHEIMERS-DISEASE BRAIN-SEROTONIN PARKINSONS-DISEASE HUMAN PLASMA DEPRESSION METABOLISM SCHIZOPHRENIA TRYPTOPHAN DISORDERS |
| Issue Date | 2015 |
| Publisher | RAPID COMMUNICATIONS IN MASS SPECTROMETRY |
| Citation | RAPID COMMUNICATIONS IN MASS SPECTROMETRY.2015,29,(16),1491-1500. |
| Abstract | RATIONALE: Although serotonin deficiency is involved with various physiological disorders such as Alzheimer's disease, Parkinson's disease, schizophrenia and depression, the serotonin-dependent pathomechanisms remain poorly understood, particularly from a lipidomics perspective. METHODS: This study therefore aimed to identify novel lipid biomarkers associated with serotonin deficiency by lipid profiling of p-chlorophenylalanine (pCPA)-treated, serotonin-deficient mice using continuous-flow normal-phase/reversed-phase two-dimensional liquid chromatography/quadrupole time-of-flight mass spectrometry (NP/RP 2D LC/QTOFMS). Principal component analysis (PCA) was performed to distinguish significantly altered lipids between the pCPA-treated mice and control mice. RESULTS: Eighteen lipid biomarkers were associated with pCPA-induced serotonin deficiency. Specifically, lipid species of lysophosphatidylethanolamine (LPE), phosphatidylethanolamine (PE), sphingomyelin (SM), galactosylceramide (GalCer), glucotosylceramide (GluCer), lactosylceramide (LacCer) and triacylglycerol (TG) were down-regulated whereas glycerophosphocholine (PC) and phosphatidylinositol (PI) were up-regulated in the pCPA-treated mice compared with control mice. CONCLUSIONS: This work demonstrates the significant effects of serotonin deficiency on lipid metabolisms and will facilitate improved understanding of pathomechanisms in serotonin deficiency, particularly from a lipidomics perspective. Copyright (C) 2015 John Wiley & Sons, Ltd. |
| URI | http://hdl.handle.net/20.500.11897/417006 |
| ISSN | 0951-4198 |
| DOI | 10.1002/rcm.7241 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | å å¦ä¸ å å å·¥ç¨ å¦é ¢ 实验动物中心 生物有机与分子工程教育部重点实验室 |
