| Title | Follicular dendritic cell-secreted protein may enhance osteoclastogenesis in periodontal disease |
| Authors | Liu, Jianru Bian, Huan Ding, Ruiyu Chi, Xiaopei Wang, Yixiang |
| Affiliation | Peking Univ, Sch & Hosp Stomatol, Dept Periodontol, Beijing 100081, Peoples R China. Chinese Peoples Liberat Army Gen Hosp, Affiliated Hosp 1, Dept Stomatol, Beijing 100853, Peoples R China. Peking Univ, Sch & Hosp Stomatol, Dept VIP Dent Serv, Beijing 100081, Peoples R China. Peking Univ, Sch & Hosp Stomatol, Cent Lab, 22 Zhongguancun Ave, Beijing 100081, Peoples R China. |
| Keywords | osteoclastogenesis lipopolysaccharide periodontal ligament cells osteogenesis FDC-SP OSTEOGENIC DIFFERENTIATION LIGAMENT CELLS TNF-ALPHA FDC-SP EXPRESSION GENES PATHOGENESIS IL-1-BETA TISSUE |
| Issue Date | 2016 |
| Publisher | CONNECTIVE TISSUE RESEARCH |
| Citation | CONNECTIVE TISSUE RESEARCH.2016,57,(1),38-43. |
| Abstract | Purpose of the study: Follicular dendritic cell-secreted protein (FDC-SP) has been found to be expressed in periodontal ligament (PDL), a layer of soft connective tissue between tooth root and alveolar bone, and involved in immunoreaction. This study was performed to explore the potential role of FDC-SP in periodontal disease. Materials and Methods: The human periodontal ligament cells (hPDLCs) were stimulated with Porphyromonas gingivalis (P. gingivalis) lipopolysaccharide (LPS) and FDC-SP expression was examined by real-time PCR and western blot. Then this molecule was overexpressed or silenced in hPDLCs by transfection of FDC-SP expression plasmids or its small-interfering (si) RNA, respectively, and the effects of FDC-SP on expression of osteogenesis- and osteoclastogenesis-related genes in hPDLCs were analyzed by real-time PCR and western blot. Results: Our results showed that P. gingivalis LPS upregulated FDC-SP expression in hPDLCs. Overexpression of FDC-SP could decrease the expression of osteogenesis-related genes, increase the expression of osteoclastogenesis-related genes and RANKL/OPG ratio in hPDLCs. Meanwhile, silence of FDC-SP expression in hPDLCs remarkably inversed the above results. Conclusions: LPS-induced upregulation of FDC-SP expression in hPDLCs may enhance osteoclastogenesis in periodontal disease. |
| URI | http://hdl.handle.net/20.500.11897/416695 |
| ISSN | 0300-8207 |
| DOI | 10.3109/03008207.2015.1095892 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 口腔医院 |
