| Title | Carbon "Quantum" Dots for Fluorescence Labeling of Cells |
| Authors | Liu, Jia-Hui Cao, Li LeCroy, Gregory E. Wang, Ping Meziani, Mohammed J. Dong, Yiyang Liu, Yuanfang Luo, Pengju G. Sun, Ya-Ping |
| Affiliation | Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing Key Lab Bioproc, Beijing 100029, Peoples R China. Clemson Univ, Dept Chem, Clemson, SC 29634 USA. Clemson Univ, Lab Emerging Mat & Technol, Clemson, SC 29634 USA. Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China. Shanghai Univ, Inst Nanochem & Nanobiol, Shanghai 200444, Peoples R China. |
| Keywords | carbon dots fluorescence labeling cell imaging stem cells two-photon fluorescence quantum dots STEM-CELLS LIVE CELLS IN-VIVO NANOPARTICLES NANODOTS PHOTOLUMINESCENCE GRAPHENE TISSUES FUNCTIONALIZATION TRACKING |
| Issue Date | 2015 |
| Publisher | ACS APPLIED MATERIALS & INTERFACES |
| Citation | ACS APPLIED MATERIALS & INTERFACES.2015,7,(34),19439-19445. |
| Abstract | The specifically synthesized and selected carbon dots of relatively high fluorescence quantum yields were evaluated in their fluorescence labeling of cells. For the cancer cell lines, the cellular uptake of the carbon dots was generally efficient, resulting in the labeling of the cells with bright fluorescence emissions for both one- and two-photon excitations from predominantly the cell membrane and cytoplasm. In the exploration on labeling the live stem cells, the cellular uptake of the carbon dots was relatively less efficient, though fluorescence emissions could still be adequately detected in the labeled cells, with the emissions again predominantly from the cell membrane and cytoplasm. This combined with the observed more efficient internalization of the same carbon dots by the fixed stem cells might suggest some significant selectivity of the stem cells toward surface functionalities of the carbon dots. The needs and possible strategies for more systematic and comparative studies on the fluorescence labeling of different cells, including especially live stem cells, by carbon dots as a new class of brightly fluorescent probes are discussed. |
| URI | http://hdl.handle.net/20.500.11897/416383 |
| ISSN | 1944-8244 |
| DOI | 10.1021/acsami.5b05665 |
| Indexed | SCI(E) EI PubMed |
| Appears in Collections: | å å¦ä¸ å å å·¥ç¨ å¦é ¢ |
