| Title | Retargeted human avidin-CAR T cells for adoptive immunotherapy of EGFRvIII expressing gliomas and their evaluation via optical imaging |
| Authors | Liu, Kaiyu Liu, Xujie Peng, Zhiping Sun, Haojie Zhang, Mingzhi Zhang, Jianning Liu, Shuang Hao, Limin Lu, Guoqiu Zheng, Kangcheng Gong, Xikui Wu, Di Wang, Fan Shen, Li |
| Affiliation | Peking Univ, Dept Biochem & Mol Biol, Hlth Sci Ctr, Beijing 100871, Peoples R China. Chongqing Med Univ, Dept Med Radiol, Chongqing, Peoples R China. Peking Univ, Dept Cell Biol, Hlth Sci Ctr, Beijing 100871, Peoples R China. Peking Univ, Stem Cell Res Ctr, Beijing 100871, Peoples R China. Chinese PLA Navy Gen Hosp, Dept Neurosurg, Beijing, Peoples R China. Beijing Cellonis Biotechnol Co Ltd, Beijing, Peoples R China. Peking Univ, Med Isotopes Res Ctr, Beijing 100871, Peoples R China. Peking Univ, Dept Radiat Med, Sch Basic Med Sci, Beijing 100871, Peoples R China. |
| Keywords | adoptive immunotherapy avidin-CAR biotinylated EGFRvIII optical imaging GROWTH-FACTOR RECEPTOR CHIMERIC ANTIGEN RECEPTOR ANTITUMOR-ACTIVITY GLIOBLASTOMA-MULTIFORME CANCER THERAPY TUMORIGENICITY NEUROBLASTOMA CONTRIBUTES LYMPHOCYTES |
| Issue Date | 2015 |
| Publisher | ONCOTARGET |
| Citation | ONCOTARGET.2015,6,(27),23735-23747. |
| Abstract | There has been significant progress in the design of chimeric antigen receptors (CAR) for adoptive immunotherapy targeting tumor-associated antigens. However, the challenge of monitoring the therapy in real time has been continually ignored. To address this issue, we developed optical molecular imaging approaches to evaluate a recently reported novel CAR strategy for adoptive immunotherapy against glioma xenografts expressing EGFRvIII. We initially biotinylated a novel anti-EGFRvIII monoclonal antibody (biotin-4G1) to pre-target EGFRvIII(+) gliomas and then redirect activated avidin-CAR expressing T cells against the pre-targeted biotin-4G1. By optical imaging study and bio-distribution analysis, we confirmed the specificity of pre-target and target and determined the optimal time for T cells adoptive transfer in vivo. The results showed this therapeutic strategy offered efficient therapy effect to EGFRvIII(+) glioma-bearing mice and implied that optical imaging is a highly useful tool in aiding in the instruction of clinical CAR-T cells adoptive transfer in future. |
| URI | http://hdl.handle.net/20.500.11897/416266 |
| ISSN | 1949-2553 |
| DOI | 10.18632/oncotarget.4362 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 基础医学院 |
