| Title | The alteration in the architecture of a T-DNA insertion rice mutant osmtd1 is caused by up-regulation of MicroRNA156f |
| Authors | Liu, Qing Shen, Gezhi Peng, Keqin Huang, Zhigang Tong, Jianhua Kabir, Mohammed Humayun Wang, Jianhui Zhang, Jingzhe Qin, Genji Xiao, Langtao |
| Affiliation | Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Peoples R China. Shanghai Acad Agr Sci, Crop Inst, Shanghai 201106, Peoples R China. Sichuan Acad Agr Sci, Inst Hort, Chengdu 610066, Peoples R China. Peking Univ, Coll Life Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China. |
| Keywords | Oryza sativa OsmiR156f plant architecture protease inhibitor T-DNA insertion PLANT ARCHITECTURE TRANSCRIPTION FACTORS NEGATIVE REGULATION PROTEASE INHIBITOR SHOOT GRAVITROPISM BUD OUTGROWTH PHASE-CHANGE MONOCULM 1 ARABIDOPSIS GENE |
| Issue Date | 2015 |
| Publisher | JOURNAL OF INTEGRATIVE PLANT BIOLOGY |
| Citation | JOURNAL OF INTEGRATIVE PLANT BIOLOGY.2015,57,(10),819-829. |
| Abstract | Plant architecture is an important factor for crop production. Some members of microRNA156 (miR156) and their target genes SQUAMOSAPromoter-Binding Protein-Like (SPL) were identified to play essential roles in the establishment of plant architecture. However, the roles and regulation of miR156 is not well understood yet. Here, we identified a T-DNA insertion mutant Osmtd1 (Oryza sativa multi-tillering and dwarf mutant). Osmtd1 produced more tillers and displayed short stature phenotype. We determined that the dramatic morphological changes were caused by a single T-DNA insertion in Osmtd1. Further analysis revealed that the T-DNA insertion was located in the gene Os08g34258 encoding a putative inhibitor I family protein. Os08g34258 was knocked out and OsmiR156f was significantly upregulated in Osmtd1. Overexpression of Os08g34258 in Osmtd1 complemented the defects of the mutant architecture, while overexpression of OsmiR156f in wild-type rice phenocopied Osmtd1. We showed that the expression of OsSPL3, OsSPL12, and OsSPL14 were significantly downregulated in Osmtd1 or OsmiR156f overexpressed lines, indicating that OsSPL3, OsSPL12, and OsSPL14 were possibly direct target genes of OsmiR156f. Our results suggested that OsmiR156f controlled plant architecture by mediating plant stature and tiller outgrowth and may be regulated by an unknown protease inhibitor I family protein. |
| URI | http://hdl.handle.net/20.500.11897/415934 |
| ISSN | 1672-9072 |
| DOI | 10.1111/jipb.12340 |
| Indexed | SCI(E) PubMed 中国科技核心期刊(ISTIC) 中国科学引文数据库(CSCD) |
| Appears in Collections: | 生命科学学院 |
