| Title | Diacylglycerol Guides the Hopping of Clathrin-Coated Pits along Microtubules for Exo-Endocytosis Coupling |
| Authors | Yuan, Tianyi Liu, Lin Zhang, Yongdeng Wei, Lisi Zhao, Shiqun Zheng, Xiaolu Huang, Xiaoshuai Boulanger, Jerome Gueudry, Charles Lu, Jingze Xie, Lihan Du, Wen Zong, Weijian Yang, Lu Salamero, Jean Liu, Yanmei Chen, Liangyi |
| Affiliation | Peking Univ, Inst Mol Med, Beijing Key Lab Cardiometabol Mol Med, State Key Lab Membrane Biol, Beijing 100871, Peoples R China. Natl Ctr Nanosci & Technol, Beijing 100871, Peoples R China. Chinese Acad Sci, Inst Biophys, Natl Key Lab Biomacromol, Beijing 100101, Peoples R China. UPMC, CNRS, Inst Curie, UMR144, F-75005 Paris, France. Inst Curie, PICT Cell & Tissue Imaging Core Facil, F-75005 Paris, France. Roper Sci SAS, F-91017 Evry, France. |
| Keywords | PANCREATIC BETA-CELLS MEDIATED ENDOCYTOSIS SYNAPTIC VESICLES PERIACTIVE ZONE ACTIVE ZONE LIVE CELLS EXOCYTOSIS MEMBRANE DYNAMIN MICROSCOPY |
| Issue Date | 2015 |
| Publisher | DEVELOPMENTAL CELL |
| Citation | DEVELOPMENTAL CELL.2015,35,(1),120-130. |
| Abstract | Many receptor-mediated endocytic processes are mediated by constitutive budding of clathrin-coated pits (CCPs) at spatially randomized sites before slowly pinching off from the plasma membrane (60100 s). In contrast, clathrin-mediated endocytosis (CME) coupled with regulated exocytosis in excitable cells occurs at peri-exocytic sites shortly after vesicle fusion (similar to 10 s). The molecular mechanism underlying this spatiotemporal coupling remains elusive. We show that coupled endocytosis makes use of preformed CCPs, which hop to nascent fusion sites nearby following vesicle exocytosis. A dynamic cortical microtubular network, anchored at the cell surface by the cytoplasmic linker-associated protein on microtubules and the LL5 beta/ELKS complex on the plasma membrane, provides the track for CCP hopping. Local diacylglycerol gradients generated upon exocytosis guide the direction of hopping. Overall, the CCP-cytoskeleton-lipid interaction demonstrated here mediates exocytosis-coupled fast recycling of both plasma membrane and vesicular proteins, and it is required for the sustained exocytosis during repetitive stimulations. |
| URI | http://hdl.handle.net/20.500.11897/415764 |
| ISSN | 1534-5807 |
| DOI | 10.1016/j.devcel.2015.09.004 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 分子医学研究所 膜生物学国家重点实验室 |
