| Title | 2-D microfluidic chemostat system for long-term tracking of E.coli in growing population with single-cell resolution |
| Authors | Xu, Lu-Ping Yao, Wei-Ye Chen, Yong Taddei, Francois Ji, Hang Ouyang, Qi |
| Affiliation | Peking Univ, Ctr Theoret Biol, Beijing, Peoples R China. |
| Keywords | 2-D culture bacteria E.coli chemostat single-cell |
| Issue Date | 2007 |
| Citation | PROCEEDINGS OF THE FIRST SHENYANG INTERNATIONAL COLLOQUIUM ON MICROFLUIDICS.. |
| Abstract | To keep bacteria growing in a monolayer is important for studying the cellular dynamics of single cells in growing population. In this paper we report on a microfluidic system for long-term '2-Dimension' culture of bacteria, which allows the bacteria to grow freely in a micro-channel without forming multilayer structure. The culture environment is well controlled to be constant and it can be easily changed from one to another if necessary. This system offers a versatile platform for many research fields such as gene expression dynamics, nois-induced diversity between individuals and familial phylogenetics. |
| URI | http://hdl.handle.net/20.500.11897/406536 |
| Indexed | CPCI-S(ISTP) |
| Appears in Collections: | 待认领 |
