| Title | One-day dietary restriction changes hepatic metabolism and potentiates the hepatotoxicity of carbon tetrachloride and chloroform in rats |
| Authors | Qin, Li-Qiang Wang, Yuan Xu, Jia-Ying Kaneko, Takashi Sato, Akio Wang, Pei-Yu |
| Affiliation | Peking Univ, Sch Publ Hlth, Dept Social Med & Hlth Educ, Beijing 100083, Peoples R China. Soochow Univ, Dept Nutr & Food Hyg, Sch Radiat Med & Publ Hlth, Suzhou, Peoples R China. Hebei Med Univ, Affiliated Hosp 4, Shijiazhuang, Peoples R China. Yamanashi Univ, Sch Med, Dept Environm Hlth, Chuo, Japan. Peking Univ, Sch Publ Hlth, Dept Social Med & Hlth Educ, Xueyuan Rd 38, Beijing 100083, Peoples R China. |
| Keywords | dietary restriction hepatotoxicity hepatic cytochrome P450 2E1 chloroform carbon tetrachloride CYTOCHROME-P450 2E1 CALORIC RESTRICTION FOOD-DEPRIVATION INDUCTION LIVER TOXICITY ETHANOL CYTOCHROMES-P450 ACETAMINOPHEN MECHANISMS |
| Issue Date | 2007 |
| Publisher | tohoku journal of experimental medicine |
| Citation | TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE.2007,212,(4),379-387. |
| Abstract | Although dietary restriction (DR) is common in modern society, research about hepatic metabolism and the hepatotoxicity induced by DR has been conducted less intensively than that induced by fasting. In the present study, we fed male Wistar rats at five levels of food intake for one day, including conventional feeding (60 kcal), three of DR (45, 30, and 15 kcal), and fasting (0 kcal), and observed the metabolic changes of hepatic cytochrome P450 2E1 (CYP2E1) and the hepatotoxicity of chloroform (CHCl3) and carbon tetrachloride (CCl4). The CYP2E1 content was significantly increased in 15 kcal-food and fasting groups. The hepatic glutathione (GSH) content, which protects the liver from hepatotoxic agents, was depleted in 15 kcal-food and fasting groups. After the challenge by CHCl3 and CCl4, the activities of aspartate aminotransferase and alanine aminotransferase, marker enzymes for liver damage, were elevated remarkably at all food groups. Moreover, their activities increased significantly in DR groups, in comparison to the corresponding 60 kcal-food group. After the challenge, the hepatic GSH content was also depleted significantly in 15 kcal-food and fasting groups. CHCl3 was cleared by hepatic metabolism about 8-10 times faster than that Of CCl4. Similarly, the areas under the blood concentration-time curve Of CCl4 was as much as twice that of the corresponding CHCl3. In conclusion, when food was restricted to less than half of conventional amount, hepatic metabolism was affected and the hepatotoxicity induced by CCl4 or CHCl3 was augmented by, at least in part, CYP2E1 induction and GSH depletion. |
| URI | http://hdl.handle.net/20.500.11897/397753 |
| ISSN | 0040-8727 |
| DOI | 10.1620/tjem.212.379 |
| Indexed | SCI(E) |
| Appears in Collections: | 公共卫生学院 |
