Title | First Report of Putative Streptococcus pneumoniae Serotype 6D among Nasopharyngeal Isolates from Fijian Children |
Authors | Jin, Ping Kong, Fanrong Xiao, Meng Oftadeh, Shahin Zhou, Fei Liu, Chunyi Russell, Fiona Gilbert, Gwendolyn L. |
Affiliation | Westmead Hosp, Ctr Infect Dis & Microbiol, Inst Clin Pathol & Med Res, Westmead, NSW 2145, Australia. Baoan Matern & Child Hlth Hosp, Shenzhen, Peoples R China. Peking Univ, Life Sci Coll, Beijing 100871, Peoples R China. Univ Melbourne, Dept Paediat, Ctr Int Child Hlth, Parkville, Vic 3052, Australia. Westmead Hosp, Ctr Infect Dis & Microbiol, Inst Clin Pathol & Med Res, Darcy Rd, Westmead, NSW 2145, Australia. |
Keywords | BLOT HYBRIDIZATION ASSAY PNEUMOCOCCAL SEROTYPE CONJUGATE VACCINE 6C PCR SEROGROUP-6 PREVALENCE DISEASE 6A |
Issue Date | 2009 |
Publisher | journal of infectious diseases |
Citation | JOURNAL OF INFECTIOUS DISEASES.2009,200,(9),1375-1380. |
Abstract | Background. A putative Streptococcus pneumoniae serotype, 6D, resulting from the introduction of wciN(beta) into serotype 6B has been proposed. Methods. We studied 98 unique serogroup 6 isolates from Fijian children, two-thirds of whom had received at least 1 dose of 7-valent pneumococcal conjugate vaccine, and 51 invasive isolates from Australian children. We used a polymerase chain reaction (PCR) system that targets both wciN(beta) and the single-nucleotide polymorphism that differentiates serotypes 6A and 6B-wciP584g (6A) and wciP584a (6B). Results. Two (9%) of 22 Australian isolates and 24 (38%) of 64 Fijian isolates previously identified as 6A by the Quellung reaction and wciP584g PCR contained wciN(beta) and were designated as 6C; 14 (41%) of 34 Fijian isolates previously identified as 6B by the Quellung reaction and wciP584a PCR contained wciN(beta) and were designated as the new putative serotype 6D. A significantly smaller proportion of children from whom serotype 6D was isolated (2/14 [14%]) had not received PCV-7, compared with the proportion of those from whom serotype 6B was isolated (11/20 [55%]) (P < .05). Conclusion. This is the first report of naturally occurring S. pneumoniae serotype 6D. |
URI | http://hdl.handle.net/20.500.11897/396223 |
ISSN | 0022-1899 |
DOI | 10.1086/606118 |
Indexed | SCI(E) |
Appears in Collections: | 生命科学学院 |