| Title | Improved identification of Gordonia, Rhodococcus and Tsukamurella species by 5 '-end 16S rRNA gene sequencing |
| Authors | Wang, Tao Kong, Fanrong Chen, Sharon Xiao, Meng Sorrell, Tania Wang, Xiaoyan Wang, Shuo Sintchenko, Vitali |
| Affiliation | Westmead Hosp, Ctr Infect Dis & Microbiol, Westmead, NSW 2145, Australia. Capital Med Univ, Beijing Tian Tan Hosp, Dept Neurosurg, Beijing, Peoples R China. Peking Univ, Life Sci Coll, Beijing 100871, Peoples R China. Inner Mongolia Med Coll, Affiliated Hosp, Dept Neurosurg, Inner Mongolia, Peoples R China. Inner Mongolia Med Coll, Affiliated Hosp, Dept Dermatol & Venereol, Inner Mongolia, Peoples R China. Univ Sydney, Sydney Med Sch, Sydney, NSW 2006, Australia. Westmead Hosp, Ctr Infect Dis & Microbiol, Darcy Rd, Westmead, NSW 2145, Australia. |
| Keywords | Gordonia Rhodococcus Tsukamurella Actinomycetes sequence based identification 16S rRNA gene reference sequence sequence polymorphisms SP NOV. COMB. NOV NOCARDIA MICROBIOLOGY ACTINOMYCETE PHYLOGENY ALIGNMENT BACTERIA STRAINS SLUDGE |
| Issue Date | 2011 |
| Publisher | pathology |
| Citation | PATHOLOGY.2011,43,(1),58-63. |
| Abstract | Objectives: The identification of fastidious aerobic Actinomycetes such as Gordonia, Rhodococcus, and Tsukamurella has remained a challenge leading to clinically significant misclassifications. This study is intended to examine the feasibility of partial 5'-end 16S rRNA gene sequencing for the identification of Gordonia, Rhodococcus, and Tsukamurella, and defined potential reference sequences for species from each of these genera. Methods: The 16S rRNA gene sequence based identification algorithm for species identification was used and enhanced by aligning test sequences with reference sequences from the List of Prokaryotic Names with Standing in Nomenclature. Results: Conventional PCR based 165 rRNA gene sequencing and the alignment of the isolate 16S rRNA gene sequence with reference sequences accurately identified 100% of clinical strains of aerobic Actinomycetes. While partial 16S rRNA gene sequences of reference type strains matched with the 16S rRNA gene sequences of 19 isolates in our data set, another 13 strains demonstrated a degree of polymorphism with a 1-4 bp difference in the regions of difference. Conclusions: 5'-end 606 bp 16S rRNA gene sequencing, coupled with the assignment of well defined reference sequences to clinically relevant species of bacteria, can be a useful strategy for improving the identification of clinically relevant aerobic Actinomycetes. |
| URI | http://hdl.handle.net/20.500.11897/395135 |
| ISSN | 0031-3025 |
| DOI | 10.1097/PAT.0b013e328340e431 |
| Indexed | SCI(E) |
| Appears in Collections: | 生命科学学院 |
