| Title | Comparison of different methods for detecting epidermal growth factor receptor mutations in peripheral blood and tumor tissue of non-small cell lung cancer as a predictor of response to gefitinib |
| Authors | Xu, Fei Wu, Jingxun Xue, Cong Zhao, Yuanyuan Jiang, Wei Lin, Liping Wu, Xuan Lu, Yachao Bai, Hua Xu, Jiasen Zhu, Guanshan Zhang, Li |
| Affiliation | Sun Yat Sen Univ, Ctr Canc, Dept Med Oncol, State Key Lab Oncol S China, Guangzhou 510060, Guangdong, Peoples R China. Xiamen Univ, Affiliated Hosp 1, Dept Med Oncol, Xiamen, Fujian, Peoples R China. Guangxi Med Univ, Canc Hosp, Dept Med Oncol, Nanning, Guangxi, Peoples R China. Guangxi Autonomous Reg Canc Hosp, Nanning, Guangxi, Peoples R China. Panyu Cent Hosp, Dept Hematol & Oncol, Guangzhou, Guangdong, Peoples R China. Sun Yat Sen Univ, Affiliated Hosp 5, Dept Chemotherapy, Zhuhai, Guangdong, Peoples R China. AstraZeneca Global R&D, Innovat Ctr China, Shanghai, Peoples R China. Peking Univ, Sch Oncol, Beijing Canc Hosp & Inst, Dept Thorac Med Oncol, Beijing 100871, Peoples R China. SurExam Biotech Co Ltd, Sci City, Guangzhou, Guangdong, Peoples R China. Sun Yat Sen Univ, Ctr Canc, Dept Med Oncol, State Key Lab Oncol S China, 651 Dongfeng Rd E, Guangzhou 510060, Guangdong, Peoples R China. |
| Keywords | non-small cell lung cancer EGFR mutation mutation detection methods gefitinib GENE-MUTATIONS SERUM ERLOTINIB EGFR DNA RESPONSIVENESS AMPLIFICATION GUIDELINES FEATURES SAMPLES |
| Issue Date | 2012 |
| Publisher | oncotargets and therapy |
| Citation | ONCOTARGETS AND THERAPY.2012,5,439-447. |
| Abstract | Background: Previous studies have reported that epidermal growth factor receptor (EGFR) mutation in tumor tissue and peripheral blood can predict the response to EGFR tyrosine kinase inhibitor (TKI) in non-small cell lung cancer (NSCLC). However, the heterogeneity of the sample sources makes it difficult to evaluate the detecting methodologies. The goal of this study is to compare different methods for analyzing EGFR mutation in blood and tumor tissue. Materials and methods: Fifty-one advanced NSCLC patients treated with gefitinib were included in the study. The EGFR mutation status of each patients' blood was analyzed by denaturing high-performance liquid chromatography (DHPLC), mutant-enriched liquidchip (ME-Liquidchip), and Scorpion Amplification Refractory Mutation System (Scorpion-ARMS) kits. EGFR mutation information in paired tumor samples detected by Scorpion-ARMS served as a reference. Comparative analyses were performed on mutation status results obtained from different methods and on the association between the clinical outcome of TKI treatment and EGFR mutation status. Results: The response rate (RR) in the whole group was 33.3%. EGFR mutation rates were identified as 15.7%, 27.5%, and 29.4% by DHPLC, ME-Liquidchip, and Scorpion-ARMS in blood, respectively. In 34 cases that had paired tumor samples, the mutation rate in tissue was 41.2%. The RRs of patients with mutation detected by different methods were 71.4% (tumor), 62.5% (blood, DHPLC), 50.0% (blood, ME-Liquidchip), and 66.7% (blood, Scorpion-ARMS). EGFR mutation detected by Scorpion-ARMS in blood and tumor tissues had better prediction of RR to EGFR-TKI (P = 0.002 and P = 0.001) than mutation detected with DHPLC and ME-Liquidchip. Conclusion: Tumor tissue sample is the best source for EGFR mutation analysis in NSCLC patients. Peripheral blood samples may be used as an alternative source only in special conditions. Scorpion-ARMS, DHPLC, or ME-Liquidchip methods are all optional for detecting tumor EGFR mutation from blood. |
| URI | http://hdl.handle.net/20.500.11897/393832 |
| ISSN | 1178-6930 |
| DOI | 10.2147/OTT.S37289 |
| Indexed | SCI(E) |
| Appears in Collections: | 北京肿瘤医院 |
