| Title | Modulation of the Differentiation of Dental Pulp Stem Cells by Different Concentrations of beta-Glycerophosphate |
| Authors | Liu, Mingyue Sun, Yao Liu, Yang Yuan, Mengtong Zhang, Zhihui Hu, Weiping |
| Affiliation | Harbin Med Coll, Dept Prosthodont, Affiliated Hosp 2, Harbin 150086, Heilongjiang, Peoples R China. Harbin Med Coll, Inst Hard Tissue Dev & Regenerat, Affiliated Hosp 2, Harbin 150086, Heilongjiang, Peoples R China. Mianyang Cent Hosp, Dept Stomatol, Mianyang 150086, Sichuan, Peoples R China. Peking Univ, Sch Stomatol, Beijing 100081, Peoples R China. |
| Keywords | MEPE DSP beta-glycerophosphate dental pulp stem cells odontoblast IN-VITRO BONE MINERALIZATION MATRIX EXPRESSION PROTEIN TISSUE GENE MEPE SIALOPHOSPHOPROTEIN |
| Issue Date | 2012 |
| Publisher | molecules |
| Citation | MOLECULES.2012,17,(2),1219-1232. |
| Abstract | Dentinogenesis is a necessary prerequisite for dental tissue engineering. One of the steps for dentinogenesis is to obtain large quantities of highly purified odontoblasts. Therefore, we have undertaken an experiment applying different concentrations of beta-glycerophosphate (beta-GP) to induce the differentiation of dental pulp stem cells (DPSCs) in a long-term 28-day culture. In the meanwhile, we have studied the time-and maturation-dependent expression of matrix extracellular phosphoglycoprotein (MEPE) and that of the odontoblast-like marker-dentin sialoprotein (DSP), in order to investigate an optimized mineralized condition. Western blot results revealed that the expression of DSP became lower when accompanied by the increase of the beta-GP concentration, and there was also an influence on MEPE expression when different concentrations of beta-GP were applied. Meanwhile, the mineralized groups had an inhibitory function on the expression of MEPE as compared with the control group. Above all, all experimental groups successfully generated mineralized nodules by Alizarin Red S and the 5 mM beta-GP group formed more mineralized nodules quantitated using the CPC extraction method. In conclusion, there is a significant modulation of the beta-GP during the differentiation of the DPSCs. The degree of odontoblast differentiation is beta-glycerophosphate concentration dependent. A low concentration of beta-GP (5 mM) has been shown to be the optimal concentration for stimulating the maturation of the DPSCs. Moreover, MEPE accompanied with DSP clearly demonstrates the degree of the differentiation. |
| URI | http://hdl.handle.net/20.500.11897/393712 |
| ISSN | 1420-3049 |
| DOI | 10.3390/molecules17021219 |
| Indexed | SCI(E) |
| Appears in Collections: | 口腔医院 |
