| Title | IBM1, a JmjC domain-containing histone demethylase, is involved in the regulation of RNA-directed DNA methylation through the epigenetic control of RDR2 and DCL3 expression in Arabidopsis |
| Authors | Fan, Di Dai, Yan Wang, Xuncheng Wang, Zhenjie He, Hang Yang, Hongchun Cao, Ying Deng, Xing Wang Ma, Ligeng |
| Affiliation | Capital Normal Univ, Coll Life Sci, Beijing 100048, Peoples R China. Natl Inst Biol Sci, Beijing 102206, Peoples R China. Beijing Normal Univ, Coll Life Sci, Beijing 100875, Peoples R China. Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China. Hebei Normal Univ, Coll Life Sci, Shijiazhuang 050016, Peoples R China. Capital Normal Univ, Coll Life Sci, 105 Xisanhuan Beilu, Beijing 100048, Peoples R China. |
| Keywords | LOCUS-SPECIFIC CONTROL CONTAINING PROTEINS PROCESSING CENTER INVERTED REPEAT HUMAN GENOME THALIANA GENES PLANTS SIRNA METHYLTRANSFERASE |
| Issue Date | 2012 |
| Publisher | 核酸研究 |
| Citation | NUCLEIC ACIDS RESEARCH.2012,40,(18),8905-8916. |
| Abstract | Small RNA-directed DNA methylation (RdDM) is an important epigenetic pathway in Arabidopsis that controls the expression of multiple genes and several developmental processes. RNA-DEPENDENT RNA POLYMERASE 2 (RDR2) and DICER-LIKE 3 (DCL3) are necessary factors in 24-nt small interfering RNA (siRNA) biogenesis, which is part of the RdDM pathway. Here, we found that Increase in BONSAI Methylation 1 (IBM1), a conserved JmjC family histone demethylase, is directly associated with RDR2 and DCL3 chromatin. The mutation of IBM1 induced the hypermethylation of H3K9 and DNA non-CG sites within RDR2 and DCL3, which repressed their expression. A genome-wide analysis suggested that the reduction in RDR2 and DCL3 expression affected siRNA biogenesis in a locus-specific manner and disrupted RdDM-directed gene repression. Together, our results suggest that IBM1 regulates gene expression through two distinct pathways: direct association to protect genes from silencing by preventing the coupling of histone and DNA methylation, and indirect silencing of gene expression through RdDM-directed repression. |
| URI | http://hdl.handle.net/20.500.11897/392829 |
| ISSN | 0305-1048 |
| DOI | 10.1093/nar/gks647 |
| Indexed | SCI(E) |
| Appears in Collections: | 生命科学学院 |
