| Title | C13C4.5/Spinster, an evolutionarily conserved protein that regulates fertility in C-elegans through a lysosome-mediated lipid metabolism process |
| Authors | Han, Mei Chang, Hao Zhang, Peng Chen, Tao Zhao, Yanhua Zhang, Yongdeng Liu, Pingsheng Xu, Tao Xu, Pingyong |
| Affiliation | Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China. Univ Chinese Acad Sci, Beijing 100049, Peoples R China. Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Peoples R China. Peking Univ, Coll Engn, Beijing 100871, Peoples R China. Peking Univ, Biodynam Opt Imaging Ctr BIOPIC, Beijing 100871, Peoples R China. Chinese Acad Sci, Inst Biophys, Key Lab Interdisciplinary Res, Beijing 100101, Peoples R China. |
| Keywords | C13C4.5 Spinster lipid droplet SRS microscopy lysosomal enlargement fertility CAENORHABDITIS-ELEGANS FLUORESCENT PROTEINS DROPLETS FAT GENOME RNAI MICROSCOPY GENES INTERFERENCE SPINSTER |
| Issue Date | 2013 |
| Publisher | protein cell |
| Citation | PROTEIN & CELL.2013,4,(5),364-372. |
| Abstract | Lipid droplets, which are conserved across almost all species, are cytoplasmic organelles used to store neutral lipids. Identification of lipid droplet regulators will be conducive to resolving obesity and other fat-associated diseases. In this paper, we selected 11 candidates that might be associated with lipid metabolism in Caenorhabditis elegans. Using a BODIPY 493/503-based flow cytometry screen, 6 negative and 3 positive regulators of fat content were identified. We selected one negative regulator of lipid content, C13C4.5, for future study. C13C4.5 was mainly expressed in the worm intestine. We found that this gene was important for maintaining the metabolism of lipid droplets. Biochemical results revealed that 50% of triacylglycerol (TAG) was lost in C13C4.5 knockout worms. Stimulated Raman scattering (SRS) signals in C13C4.5 mutants showed only 49.6% of the fat content in the proximal intestinal region and 86.3% in the distal intestinal region compared with wild type animals. The mean values of lipid droplet size and intensity in C13C4.5 knockout animals were found to be significantly decreased compared with those in wild type worms. The LMP-1-labeled membrane structures in worm intestines were also enlarged in C13C4.5 mutant animals. Finally, fertility defects were found in C13C4.5(ok2087) mutants. Taken together, these results indicate that C13C4.5 may regulate the fertility of C. elegans by changing the size and fat content of lipid droplets by interfering with lysosomal morphology and function. |
| URI | http://hdl.handle.net/20.500.11897/391667 |
| ISSN | 1674-800X |
| DOI | 10.1007/s13238-013-3015-z |
| Indexed | SCI(E) 中国科学引文数据库(CSCD) |
| Appears in Collections: | 工学院 生物医学前沿创新中心 |
