| Title | Tunable lifetime multiplexing using luminescent nanocrystals |
| Authors | Lu, Yiqing Zhao, Jiangbo Zhang, Run Liu, Yujia Liu, Deming Goldys, Ewa M. Yang, Xusan Xi, Peng Sunna, Anwar Lu, Jie Shi, Yu Leif, Robert C. Huo, Yujing Shen, Jian Piper, James A. Robinson, J. Paul Jin, Dayong |
| Affiliation | Macquarie Univ, MQ Photon Res Ctr, Adv Cytometry Labs, Sydney, NSW 2109, Australia. Macquarie Univ, MQ BioFocus Res Ctr, Adv Cytometry Labs, Sydney, NSW 2109, Australia. Macquarie Univ, Dept Chem & Biomol Sci, Sydney, NSW 2109, Australia. Peking Univ, Coll Engn, Dept Biomed Engn, Beijing 100871, Peoples R China. Shanghai Jiao Tong Univ, Sch Biomed Engn, Shanghai 200030, Peoples R China. Newport Instruments, San Diego, CA 92117 USA. Tsinghua Univ, Dept Elect Engn, Beijing 100084, Peoples R China. Olympus Australia, N Ryde, NSW 2113, Australia. Purdue Univ, Bindley Biosci Ctr, Cytometry Labs, W Lafayette, IN 47907 USA. |
| Keywords | GENE-EXPRESSION PATTERNS UP-CONVERSION IMAGING MICROSCOPY FLOW-CYTOMETRY DNA NANOPARTICLES CELL SIZE MICROARRAY RESOLUTION |
| Issue Date | 2014 |
| Publisher | 自然光电学 |
| Citation | NATURE PHOTONICS.2014,8,(1),33-37. |
| Abstract | Optical multiplexing plays an important role in applications such as optical data storage(1), document security(2), molecular probes(3,4) and bead assays for personalized medicine(5). Conventional fluorescent colour coding is limited by spectral overlap and background interference, restricting the number of distinguishable identities. Here, we show that tunable luminescent lifetimes tau in the microsecond region can be exploited to code individual upconversion nanocrystals. In a single colour band, one can generate more than ten nanocrystal populations with distinct lifetimes ranging from 25.6 mu s to 662.4 mu s and decode their well-separated lifetime identities, which are independent of both colour and intensity. Such 'tau-dots' potentially suit multichannel bioimaging, high-throughput cytometry quantification, high-density data storage, as well as security codes to combat counterfeiting. This demonstration extends the optical multiplexing capability by adding the temporal dimension of luminescent signals, opening new opportunities in the life sciences, medicine and data security. |
| URI | http://hdl.handle.net/20.500.11897/390619 |
| ISSN | 1749-4885 |
| DOI | 10.1038/NPHOTON.2013.322 |
| Indexed | SCI(E) EI |
| Appears in Collections: | 工学院 |
