| Title | CasOT: a genome-wide Cas9/gRNA off-target searching tool |
| Authors | Xiao, An Cheng, Zhenchao Kong, Lei Zhu, Zuoyan Lin, Shuo Gao, Ge Zhang, Bo |
| Affiliation | Peking Univ, Minist Educ, Key Lab Cell Proliferat & Differentiat, Beijing 100871, Peoples R China. Peking Univ, State Key Lab Prot & Plant Gene Res, Ctr Bioinformat, Coll Life Sci, Beijing 100871, Peoples R China. Univ Calif Los Angeles, Dept Mol Cell & Dev Biol, Los Angeles, CA 90095 USA. |
| Keywords | SPECIFICITY SYSTEMS ENDONUCLEASE NUCLEASES |
| Issue Date | 2014 |
| Publisher | bioinformatics |
| Citation | BIOINFORMATICS.2014,30,(8),1180-1182. |
| Abstract | The CRISPR/Cas or Cas9/guide RNA system is a newly developed, easily engineered and highly effective tool for gene targeting; it has considerable off-target effects in cultured human cells and in several organisms. However, the Cas9/guide RNA target site is too short for existing alignment tools to exhaustively and effectively identify potential off-target sites. CasOT is a local tool designed to find potential off-target sites in any given genome or user-provided sequence, with user-specified types of protospacer adjacent motif, and number of mismatches allowed in the seed and non-seed regions. |
| URI | http://hdl.handle.net/20.500.11897/389516 |
| ISSN | 1367-4803 |
| DOI | 10.1093/bioinformatics/btt764 |
| Indexed | SCI(E) |
| Appears in Collections: | 细胞增殖分化调控机理研究教育部重点实验室 生命科学学院 |
