| Title | PHYTOCHROME INTERACTING FACTOR1 Enhances the E3 Ligase Activity of CONSTITUTIVE PHOTOMORPHOGENIC1 to Synergistically Repress Photomorphogenesis in Arabidopsis |
| Authors | Xu, Xiaosa Paik, Inyup Zhu, Ling Bu, Qingyun Huang, Xi Deng, Xing Wang Huq, Enamul |
| Affiliation | Univ Texas Austin, Dept Mol Biosci, Austin, TX 78712 USA. Univ Texas Austin, Inst Cellular & Mol Biol, Austin, TX 78712 USA. Peking Univ, Coll Life Sci, Peking Yale Joint Ctr Plant Mol Genet & Agrobiote, State Key Lab Prot & Plant Gene Res,Peking Tsingh, Beijing 100871, Peoples R China. Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA. |
| Keywords | NEGATIVE REGULATOR TRANSCRIPTION FACTORS UBIQUITIN LIGASE PLANT DEVELOPMENT PHOTOACTIVATED PHYTOCHROMES MEDIATED DEGRADATION SEED-GERMINATION LIGHT SIGNALS RED-LIGHT COP1 |
| Issue Date | 2014 |
| Publisher | plant cell |
| Citation | PLANT CELL.2014,26,(5),1992-2006. |
| Abstract | CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1) is a RING/WD40 repeat-containing ubiquitin E3 ligase that is conserved from plants to humans. COP1 forms complexes with SUPPRESSOR OF PHYTOCHROME A (SPA) proteins, and these complexes degrade positively acting transcription factors in the dark to repress photomorphogenesis. Phytochrome-interacting basic helix-loop-helix transcription factors (PIFs) also repress photomorphogenesis in the dark. In response to light, the phytochrome family of sensory photoreceptors simultaneously inactivates COP1-SPA complexes and induces the rapid degradation of PIFs to promote photomorphogenesis. However, the functional relationship between PIFs and COP1-SPA complexes is still unknown. Here, we present genetic evidence that the pif and cop1/spa Arabidopsis thaliana mutants synergistically promote photomorphogenesis in the dark. LONG HYPOCOTYL5 (HY5) is stabilized in the cop1 pif1, spa123 pif1, and pif double, triple, and quadruple mutants in the dark. Moreover, the hy5 mutant suppresses the constitutive photomorphogenic phenotypes of the pifq mutant in the dark. PIF1 forms complexes with COP1, HY5, and SPA1 and enhances the substrate recruitment and autoubiquitylation and transubiquitylation activities of COP1. These data uncover a novel function of PIFs as the potential cofactors of COP1 and provide a genetic and biochemical model of how PIFs and COP1-SPA complexes synergistically repress photomorphogenesis in the dark. |
| URI | http://hdl.handle.net/20.500.11897/389359 |
| ISSN | 1040-4651 |
| DOI | 10.1105/tpc.114.125591 |
| Indexed | SCI(E) |
| Appears in Collections: | 生命科学学院 |
