| Title | OsMYB103L, an R2R3-MYB transcription factor, influences leaf rolling and mechanical strength in rice (Oryza sativa L.) |
| Authors | Yang, Chunhua Li, Dayong Liu, Xue Ji, Chengjun Hao, Lili Zhao, Xianfeng Li, Xiaobing Chen, Caiyan Cheng, Zhukuan Zhu, Lihuang |
| Affiliation | Chinese Acad Sci, State Key Lab Plant Genom, Beijing 100101, Peoples R China. Chinese Acad Sci, Natl Ctr Plant Gene Res, Inst Genet & Dev Biol, Beijing 100101, Peoples R China. Chinese Acad Sci, Beijing Inst Gen, CAS Key Lab Genome Sci & Informat, Beijing 100101, Peoples R China. Peking Univ, Dept Ecol, Beijing 100871, Peoples R China. Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Hunan, Peoples R China. |
| Keywords | OsMYB103L Leaf rolling MYB transcription factor Cellulose SECONDARY CELL-WALL ARABIDOPSIS-THALIANA GENE FAMILY CELLULOSE SYNTHASE HIGHER-PLANTS MYB-GENE BIOSYNTHETIC-PATHWAY EXPRESSION ANALYSIS CATALYTIC SUBUNIT TRANSGENIC RICE |
| Issue Date | 2014 |
| Publisher | bmc plant biology |
| Citation | BMC PLANT BIOLOGY.2014,14. |
| Abstract | Background: The shape of grass leaves possesses great value in both agronomy and developmental biology research. Leaf rolling is one of the important traits in rice (Oryza sativa L.) breeding. MYB transcription factors are one of the largest gene families and have important roles in plant development, metabolism and stress responses. However, little is known about their functions in rice. Results: In this study, we report the functional characterization of a rice gene, OsMYB103L, which encodes an R2R3-MYB transcription factor. OsMYB103L was localized in the nucleus with transactivation activity. Overexpression of OsMYB103L in rice resulted in a rolled leaf phenotype. Further analyses showed that expression levels of several cellulose synthase genes (CESAs) were significantly increased, as was the cellulose content in OsMYB103L overexpressing lines. Knockdown of OsMYB103L by RNA interference led to a decreased level of cellulose content and reduced mechanical strength in leaves. Meanwhile, the expression levels of several CESA genes were decreased in these knockdown lines. Conclusions: These findings suggest that OsMYB103L may target CESA genes for regulation of cellulose synthesis and could potentially be engineered for desirable leaf shape and mechanical strength in rice. |
| URI | http://hdl.handle.net/20.500.11897/389040 |
| ISSN | 1471-2229 |
| DOI | 10.1186/1471-2229-14-158 |
| Indexed | SCI(E) |
| Appears in Collections: | 城市与环境学院 |
