Title | Presence of Stem/Progenitor Cells in the Rat Penis |
Authors | Lin, Guiting Alwaal, Amjad Zhang, Xiaoyu Wang, Jianwen Wang, Lin Li, Huixi Wang, Guifang Ning, Hongxiu Lin, Ching-Shwun Xin, Zhongcheng Lue, Tom F. |
Affiliation | Univ Calif San Francisco, Sch Med, Dept Urol, Knuppe Mol Urol Lab, San Francisco, CA 94143 USA. Peking Univ, Hosp 1, Dept Urol, Beijing 100871, Peoples R China. Peking Univ, Inst Urol, Beijing 100871, Peoples R China. Capital Med Univ, Sch Med, Dept Urol, Beijing, Peoples R China. Univ Calif San Francisco, Sch Med, Dept Urol, Knuppe Mol Urol Lab, 400 Parnassus Ave,Suite A-630, San Francisco, CA 94143 USA. |
Keywords | LABEL-RETAINING CELLS STEM-CELLS ERECTILE FUNCTION PROGENITOR CELLS DIFFERENTIATION PROLIFERATION BRDU DISEASE SKIN |
Issue Date | 2015 |
Publisher | 干细胞与发育 |
Citation | STEM CELLS AND DEVELOPMENT.2015,24,(2),264-270. |
Abstract | Tissue resident stem cells are believed to exist in every organ, and their identification is commonly done using a combination of immunostaining for putative stem cell markers and label-retaining cell (LRC) strategy. In this study, we employed these approaches to identify potential stem cells in the penis. Newborn rats were intraperitoneally injected with thymidine analog, 5-ethynyl-2-deoxyuridine (EdU), and their penis was harvested at 7 h, 3 days, 1 week, and 4 weeks. It was processed for EdU stains and immunofluorescence staining for stem cell markers A2B5, PCNA, and c-kit. EdU-positive cells were counted for each time point and co-localized with each stem cell marker, then isolated and cultured in vitro followed by their characterization using flowcytometry and immunofluorescence. At 7 h post-EdU injection, 410 +/- 105.3 penile corporal cells were labeled in each cross-section (similar to 28%). The number of EdU-positive cells at 3 days increased to 536 +/- 115.6, while their percentage dropped to 25%. Progressively fewer EdU-positive cells were present in the sacrificed rat penis at longer time points (1 and 4 weeks). They were mainly distributed in the subtunic and perisinusoidal spaces, and defined as subtunic penile progenitor cells (STPCs) and perisinusoidal penile progenitor cells (PPCs). These cells expressed c-kit, A2B5, and PCNA. After culturing in vitro, only similar to 0.324% corporal cells were EdU-labeled LRCs and expressed A2B5/PCNA. Therefore, labeling of penis cells by EdU occurred randomly, and label retaining was not associated with expression of c-kit, A2B5, or PCNA. The penile LRCs are mainly distributed within the subtunic and perisinusoidal space. |
URI | http://hdl.handle.net/20.500.11897/386065 |
ISSN | 1547-3287 |
DOI | 10.1089/scd.2014.0360 |
Indexed | SCI(E) |
Appears in Collections: | 第一医院 |