| Title | Immunoglobulin mRNA and protein expression in human oral epithelial tumor cells |
| Authors | Zhu, Xiaohui Li, Cuiying Sun, Xin Mao, Yuntao Li, Guohui Litt, Xiaoyong Zhang, Youhui Qiu, Xiaoyan |
| Affiliation | Peking Univ, Ctr Human Dis Genom, Beijing 100083, Peoples R China. Capital Univ Med Sci, Beijing Stomatol Hosp, Dept Pathol, Beijing, Peoples R China. Chinese Acad Med Sci, Dept Immunol, Inst Canc, Beijing 100037, Peoples R China. Peking Union Med Coll, Beijing 100021, Peoples R China. Peking Univ, Ctr Human Dis Genom, 38 Xueyuan Rd, Beijing 100083, Peoples R China. |
| Keywords | immunoglobulin expression V(D)J rearrangement oral epithelial tumor CHAIN CONSTANT-REGION BREAST-CANCER PATIENT PLASMA LINES |
| Issue Date | 2008 |
| Publisher | applied immunohistochemistry molecular morphology |
| Citation | APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY.2008,16,(3),232-238. |
| Abstract | In our previous work, we have reported the expression of immunoglobulin (Ig) molecules by numerous epithelial cancer cells and hyperplastic epithelial cells. In the present study, we extended our investigation to study the frequencies of expression of IgG and IgA in some types of oral epithelial tumor cells, and analyzed the oral tumor-derived V regions characteristic of Ig gamma chain gene transcripts by immunohistochemistry, in situ hybridization, laser capture microdissection-correlated reverse-transcription polymerase chain reaction, and sequencing. IgG and IgA immunoreactivity was prominent in the cytoplasmic or plasma membrane or secretion of malignant cells, pleomorphic adenoma tumor cells, and some normal glandular epithelial cells or squamous cells adjacent to tumors. More importantly, rearranged Ig gene transcripts were identified in these tumor cells, and in some normal glandular epithelial cells, the V-DJ region sequences revealed that IgG transcripts in 2 tested oral tumors were oligoclonal. These results support that the phenomenon of Ig could also be expressed in oral cavity epithelial tumor cells. |
| URI | http://hdl.handle.net/20.500.11897/346032 |
| ISSN | 1062-3345 |
| DOI | 10.1097/PAI.0b013e31814c915a |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 待认领 |
