| Title | Coexpression of Pdx1 and betacellulin in mesenchymal stem cells could promote the differentiation of nestin-positive epithelium-like progenitors and pancreatic islet-like spheroids |
| Authors | Li, Lisha Li, Furong Qi, Hui Feng, Gao Yuan, Kehu Deng, Hongkui Zhou, Hanxin |
| Affiliation | Peking Univ, Coll Life Sci, Dept Cell Biol & Genet, Beijing 100871, Peoples R China. Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Clin Med Res Ctr, Shenzhen, Peoples R China. |
| Keywords | INSULIN-PRODUCING CELLS IN-VITRO MULTIPOTENTIAL NESTIN PRECURSOR CELLS TRANSPLANTATION EXPRESSION GROWTH MANIPULATION ENDOCRINE SECRETION |
| Issue Date | 2008 |
| Publisher | 干细胞与发育 |
| Citation | STEM CELLS AND DEVELOPMENT.2008,17,(4),815-823. |
| Abstract | Mesenchymal stem cells (MSCs) have already been proved to be multipotent. Our goal was to evaluate the differentiating ability of rat MSCs into insulin-secreting cells in vitro to cure diabetes resulting from abnormal function of pancreatic islets. MSCs were identified by Euorescence-activated cell sorting (FACS). Pdx1 is a transcription factor involved in the early endocrine development. Betacellulin (BTC) is a growth factor involved in beta-cell maturation. MSCs were transfected with plasmids carrying rat Pdx1 and BTC genes. Coexpression of Pdx1 and BTC significantly increased the number of nestin-positive epithelium-like progenitors and islet-like spheroids which differentiated from MSCs. In Pdx1- and BTC-expressed (Pdx1(+) + BTC+) MSCs, insulin and Glut-2 mRNA levels significantly rose. The number of islet-like cells was also evidently augmented. In response to glucose, Pdx1(+) + BTC+ MSCs released insulin and C-peptide. It is concluded that genetic manipulation of transcription factor Pdx1 and growth factor BTC in combination with appropriate differentiating culture could induce MSCs into the pancreatic lineage in vitro and produce islet-like spheroids that could secrete increased levels of insulin in response to glucose. |
| URI | http://hdl.handle.net/20.500.11897/345237 |
| ISSN | 1547-3287 |
| DOI | 10.1089/scd.2008.0060 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 生命科学学院 |
