| Title | Calcium Triggers Exocytosis from Two Types of Organelles in a Single Astrocyte |
| Authors | Liu, Tao Sun, Lei Xiong, Yingfei Shang, Shujiang Guo, Ning Teng, Sasa Wang, Yeshi Liu, Bin Wang, Changhe Wang, Li Zheng, Lianghong Zhang, Claire Xi Han, Weiping Zhou, Zhuan |
| Affiliation | Peking Univ, Inst Mol Med, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100871, Peoples R China. Agcy Sci Technol & Res, Singapore Bioimaging Consortium, Lab Metab Med, Singapore 138667, Singapore. Peking Univ, Inst Mol Med, State Key Lab Biomembrane & Membrane Biotechnol, 5 Yiheyuan Rd, Beijing 100871, Peoples R China. |
| Keywords | DEPENDENT GLUTAMATE RELEASE LONG-TERM POTENTIATION SYNAPTIC VESICLES VESICULAR COMPARTMENT REGULATED EXOCYTOSIS LYSOSOMAL EXOCYTOSIS CA2+ SENSOR D-SERINE RECEPTOR FUSION |
| Issue Date | 2011 |
| Publisher | journal of neuroscience |
| Citation | JOURNAL OF NEUROSCIENCE.2011,31,(29),10593-10601. |
| Abstract | Astrocytes release a variety of signaling molecules including glutamate, D-serine, and ATP in a regulated manner. Although the functions of these molecules, from regulating synaptic transmission to controlling specific behavior, are well documented, the identity of their cellular compartment(s) is still unclear. Here we set out to study vesicular exocytosis and glutamate release in mouse hippocampal astrocytes. We found that small vesicles and lysosomes coexisted in the same freshly isolated or cultured astrocytes. Both small vesicles and lysosome fused with the plasma membrane in the same astrocytes in a Ca2+-regulated manner, although small vesicles were exocytosed more efficiently than lysosomes. Blockade of the vesicle glutamate transporter or cleavage of synaptobrevin 2 and cellubrevin (both are vesicle-associated membrane proteins) with a clostridial toxin greatly inhibited glutamate release from astrocytes, while lysosome exocytosis remained intact. Thus, both small vesicles and lysosomes contribute to Ca2+-dependent vesicular exocytosis, and small vesicles support glutamate release from astrocytes. |
| URI | http://hdl.handle.net/20.500.11897/343642 |
| ISSN | 0270-6474 |
| DOI | 10.1523/JNEUROSCI.6401-10.2011 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 分子医学研究所 |
