| Title | MicroRNA-210 Contributes to Preeclampsia by Downregulating Potassium Channel Modulatory Factor 1 |
| Authors | Luo, Rongcan Shao, Xuan Xu, Peng Liu, Yanlei Wang, Yongqing Zhao, Yangyu Liu, Ming Ji, Lei Li, Yu-xia Chang, Cheng Qiao, Jie Peng, Chun Wang, Yan-ling |
| Affiliation | Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China. Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China. Peking Univ, Hosp 3, Dept Obstet & Gynecol, Beijing 100871, Peoples R China. York Univ, Dept Biol, Toronto, ON M3J 1P3, Canada. Univ Chinese Acad Sci, Beijing, Peoples R China. Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, 1 Beichen West Rd, Beijing 100101, Peoples R China. |
| Keywords | KCMF1 miR-210 placenta preeclampsia TNF-a NECROSIS-FACTOR-ALPHA TNF-ALPHA GENE-EXPRESSION TROPHOBLAST INVASION OXIDATIVE STRESS HYPOXIA PREGNANCY MIR-210 PLACENTA CANCER |
| Issue Date | 2014 |
| Publisher | hypertension |
| Citation | HYPERTENSION.2014,64,(4),839-+. |
| Abstract | Preeclampsia is a pregnancy-specific syndrome manifested by the onset of hypertension and proteinuria after the 20th week of gestation. Abnormal placenta development has been generally accepted as the initial cause of the disorder. Recently, microRNA-210 (miR-210) has been found to be upregulated in preeclamptic placentas compared with normal placentas, indicating a possible association of this small molecule with the placental pathology of preeclampsia. However, the function of miR-210 in the development of the placenta remains elusive. The aim of this study was to characterize the molecular mechanism of preeclampsia development by examining the role of miR-210. In this study, miR-210 and potassium channel modulatory factor 1 (KCMF1) expressions were compared in placentas from healthy pregnant individuals and patients with preeclampsia, and the role of miR-210 in trophoblast cell invasion via the downregulation of KCMF1 was investigated in the immortal trophoblast cell line HTR8/SVneo. The levels of KCMF1 were significantly lower in preeclamptic placenta tissues than in gestational week-matched normal placentas, which was inversely correlated with the level of miR-210. KCMF1 was validated as the direct target of miR-210 using real-time polymerase chain reaction, Western blotting, and dual luciferase assay in HTR8/SVneo cells. miR-210 inhibited the invasion of trophoblast cells, and this inhibition was abrogated by the overexpression of KCMF1. The inflammatory factor tumor necrosis factor-a could upregulate miR-210 while suppressing KCMF1 expression in HTR8/SVneo cells. This is the first report on the function of KCMF1 in human placental trophoblast cells, and the data indicate that aberrant miR-210 expression may contribute to the occurrence of preeclampsia by interfering with KCMF1-mediated signaling in the human placenta. |
| URI | http://hdl.handle.net/20.500.11897/342312 |
| ISSN | 0194-911X |
| DOI | 10.1161/HYPERTENSIONAHA.114.03530 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 第三医院 |
