| Title | MicroRNA Profiling in Chinese Patients with Primary Sjogren Syndrome Reveals Elevated miRNA-181a in Peripheral Blood Mononuclear Cells |
| Authors | Peng, Linyi Ma, Weizhi Yi, Fan Yang, Yun-Jiao Lin, Wei Chen, Hua Zhang, Xuan Zhang, Li-He Zhang, Fengchun Du, Quan |
| Affiliation | Beijing Union Med Coll Hosp, Dept Rheumatol & Clin Immunol, Beijing, Peoples R China. Chinese Acad Med Sci, Beijing 100730, Peoples R China. Peking Union Med Coll, Beijing 100021, Peoples R China. Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100871, Peoples R China. Minist Educ, Key Lab Rheumatol & Clin Immunol, Beijing, Peoples R China. Peking Univ, Beijing 100191, Peoples R China. |
| Keywords | SJOGREN SYNDROME MICRORNA-181A T CELL MATURATION CLASSIFICATION CRITERIA EXPRESSION PATHOGENESIS DISEASE |
| Issue Date | 2014 |
| Publisher | 风湿病学杂志 |
| Citation | JOURNAL OF RHEUMATOLOGY.2014,41,(11),2208-2213. |
| Abstract | Objective. Characterized by chronic inflammation, dysfunction of exocrine glands, and systemic autoimmunity, primary Sjogren syndrome (pSS) is a common autoimmune disease in elderly women. Our study was performed to explore the potential involvement of microRNA (miRNA) in Chinese patients with pSS. Methods. Using microarrays, miRNA expression in peripheral blood mononuclear cells (PBMC) was profiled in 4 female patients with pSS and 3 healthy participants, followed by a large-scale study of 33 patients and 10 healthy individuals. Compared to the healthy participants, 202 miRNA were upregulated and 180 were downregulated in the patients with pSS. To confirm this finding, a set of regulated miRNA was further examined in a large patient group, using quantitative reverse transcriptase-PCR assays. Results. MiR-181a was the miRNA that most profoundly differed between patients with pSS and healthy individuals; however, similar miRNA-181a expression profiles were found in groups with different disease phenotypes. Together, these observations suggested that an elevated miRNA-181a level is a general phenomenon in Chinese patients with pSS. Conclusion. In addition to the elevated miR-181a levels, our study led to the speculation that elevated miR-181a levels in the PBMC of these patients compromise the maturation of B cells, enabling them to recognize and attack autoantigens and resulting in disease phenotypes. In addition to the regulation of human miRNA, many virus-derived miRNA were unexpectedly upregulated in the patients with pSS, suggesting that viral infection of PBMC plays a role in this disease. |
| URI | http://hdl.handle.net/20.500.11897/342282 |
| ISSN | 0315-162X |
| DOI | 10.3899/jrheum.131154 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 药学院 天然药物与仿生药物国家重点实验室 |
