| Title | De novo assembly of soybean wild relatives for pan-genome analysis of diversity and agronomic traits |
| Authors | Li, Ying-hui Zhou, Guangyu Ma, Jianxin Jiang, Wenkai Jin, Long-guo Zhang, Zhouhao Guo, Yong Zhang, Jinbo Sui, Yi Zheng, Liangtao Zhang, Shan-shan Zuo, Qiyang Shi, Xue-hui Li, Yan-fei Zhang, Wan-ke Hu, Yiyao Kong, Guanyi Hong, Hui-long Tan, Bing Song, Jian Liu, Zhang-xiong Wang, Yaoshen Ruan, Hang Yeung, Carol K. L. Liu, Jian Wang, Hailong Zhang, Li-juan Guan, Rong-xia Wang, Ke-jing Li, Wen-bin Chen, Shou-yi Chang, Ru-zhen Jiang, Zhi Jackson, Scott A. Li, Ruiqiang Qiu, Li-juan |
| Affiliation | Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100193, Peoples R China. Chinese Acad Agr Sci, Inst Crop Sci, MOA, Key Lab Crop Gene Resource & Germplasm Enhancemen, Beijing 100193, Peoples R China. Chinese Acad Agr Sci, Inst Crop Sci, MOA, Key Lab Soybean Biol Beijing, Beijing 100193, Peoples R China. Novogene Bioinformat Inst, Beijing, Peoples R China. Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing, Peoples R China. Northeast Agr Univ, Key Lab Soybean Biol, Chinese Minist Educ, Harbin, Peoples R China. Univ Georgia, Ctr Appl Genet Technol, Athens, GA 30602 USA. Peking Univ, Biodynam Opt Imaging Ctr, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China. Peking Univ, Sch Life Sci, Beijing 100871, Peoples R China. |
| Keywords | MAXIMUM-LIKELIHOOD DISEASE RESISTANCE GENETIC DIVERSITY HIGH-THROUGHPUT OPEN SOFTWARE MOSAIC-VIRUS GLYCINE-SOJA SEQUENCE ALIGNMENT ARABIDOPSIS |
| Issue Date | 2014 |
| Publisher | nature biotechnology |
| Citation | NATURE BIOTECHNOLOGY.2014,32,(10),1045-+. |
| Abstract | Wild relatives of crops are an important source of genetic diversity for agriculture, but their gene repertoire remains largely unexplored. We report the establishment and analysis of a pan-genome of Glycine soja, the wild relative of cultivated soybean Glycine max, by sequencing and de novo assembly of seven phylogenetically and geographically representative accessions. Intergenomic comparisons identified lineage-specific genes and genes with copy number variation or large-effect mutations, some of which show evidence of positive selection and may contribute to variation of agronomic traits such as biotic resistance, seed composition, flowering and maturity time, organ size and final biomass. Approximately 80% of the pan-genome was present in all seven accessions (core), whereas the rest was dispensable and exhibited greater variation than the core genome, perhaps reflecting a role in adaptation to diverse environments. This work will facilitate the harnessing of untapped genetic diversity from wild soybean for enhancement of elite cultivars. |
| URI | http://hdl.handle.net/20.500.11897/342218 |
| ISSN | 1087-0156 |
| DOI | 10.1038/nbt.2979 |
| Indexed | SCI(E) EI PubMed |
| Appears in Collections: | 生物医学前沿创新中心 生命科学学院 |
