| Title | Identification of miR-125a-5p as a tumor suppressor of renal cell carcinoma, regulating cellular proliferation, migration and apoptosis |
| Authors | Chen, Duqun Li, Yifan Su, Zhengming Yu, Zuhu Yu, Wenshui Li, Yuchi Gui, Yaoting Yang, Shangqi Lai, Yongqing |
| Affiliation | Peking Univ, Dept Urol, Shenzhen Hosp, Shenzhen 518036, Guangdong, Peoples R China. Peking Univ, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Shenzhen Hosp, Inst Urol Shenzhen PKU HKUST Med Ctr, Shenzhen 518036, Guangdong, Peoples R China. Anhui Med Univ, Dept Urol, Hefei 230032, Anhui, Peoples R China. Shantou Univ, Dept Urol, Coll Med, Shantou 515041, Guangdon, Peoples R China. Peking Univ, Dept Urol, Shenzhen Hosp, Inst Urol Shenzhen PKU HKUST Med Ctr, 1120 Lianhua Rd, Shenzhen 518036, Guangdong, Peoples R China. |
| Keywords | miR-125a-5p tumor suppressor renal cell carcinoma BREAST-CANCER DOWN-REGULATION GASTRIC-CANCER MICRORNA PERTUZUMAB SERUM |
| Issue Date | 2015 |
| Publisher | molecular medicine reports |
| Citation | MOLECULAR MEDICINE REPORTS.2015,11,(2),1278-1283. |
| Abstract | miR-125a-5p has been previously described as a tumor suppressor in numerous malignancies, however the expression and function of miR-125a-5p in renal cell carcinoma (RCC) remains to be elucidated. In the present study, to explore the potential role of miR-125a-5p in RCC, quantitative polymerase chain reaction was used to determine the expression levels of miR-125a-5p in renal cancer tissues. The influence of miR-125a-5p on cell proliferation, migration and apoptosis was also determined, using an MTT assay, a wound scratch assay and flow cytometry, respectively. The expression of miR-125a-5p was shown to be decreased in RCC and the restoration of miR-125a-5p by synthetic mimics was shown to suppress cell proliferation and migration, and induce apoptosis. The present results indicate that miR-125a-5p may function as a tumor suppressor in RCC. The present study is, to the best of our knowledge, the first to demonstrate the downregulation of miR-125a-5p in RCC, and to show the role it has in affecting cellular proliferation, migration and apoptosis. Further research is needed to define the target genes of miR-125a-5p and explore the potential of miR-125a-5p as a diagnostic or a prognostic biomarker for RCC. |
| URI | http://hdl.handle.net/20.500.11897/341632 |
| ISSN | 1791-2997 |
| DOI | 10.3892/mmr.2014.2848 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 深圳医院 |
