| Title | Cumulus removal and single mammalian oocyte trapping on a microfluidic device |
| Authors | Han, Chao Ma, Rui Sun, Zilong Yu, Zhongyao Huang, Guoliang Zhou, Yuxiang Qiao, Jie Wang, Jundong Cheng, Jing |
| Affiliation | Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China National Engineering Research Center for Beijing Biochip Technology, 18 Life Science Parkway, Beijing 102206, China Shanxi Key Laboratory of Ecological Animal Science and Environmental Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, China Center of Reproduction Medicine, Department of Obstetrics and Gynecology, Third Hospital of Peking University, Beijing 100083, China |
| Issue Date | 2009 |
| Citation | 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009.Jeju, Korea, Republic of. |
| Abstract | We report an improved method to remove cumulus cells from cumulus-oocyte complexes (COCs) and subsequently capture individual denuded oocytes using a microfluidic device with the flow driven by a combination of negative pressure and gravity. The device consists of two poly-dimethylsiloxane (PDMS) layers, forming a digesting chamber and a pit-array area. COCs are first removed of cumulus cells by hyaluronidase in the digesting chamber, then single denuded oocytes are transported and lodged in individual pits. Multiple COCs can be concurrently processed in the microfluidic cumulus removal (CR) device to produce individual denuded oocytes for subsequent fertilization. ? 2009 CBMS. |
| URI | http://hdl.handle.net/20.500.11897/327523 |
| Indexed | EI |
| Appears in Collections: | 第三医院 |
