| Title | Pluripotent Stem Cells Induced from Mouse Somatic Cells by Small-Molecule Compounds |
| Authors | Hou, Pingping Li, Yanqin Zhang, Xu Liu, Chun Guan, Jingyang Li, Honggang Zhao, Ting Ye, Junqing Yang, Weifeng Liu, Kang Ge, Jian Xu, Jun Zhang, Qiang Zhao, Yang Deng, Hongkui |
| Affiliation | Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China. Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China. Peking Univ, Shenzhen Grad Sch, Sch Chem Biol & Biotechnol, Shenzhen 518055, Peoples R China. Beijing Vitalstar Biotechnol Co Ltd, Beijing 100012, Peoples R China. |
| Keywords | S-ADENOSYLHOMOCYSTEINE HYDROLASE REGENERATIVE MEDICINE STATE |
| Issue Date | 2013 |
| Publisher | science |
| Citation | SCIENCE.2013,341,(6146),651-654. |
| Abstract | Pluripotent stem cells can be induced from somatic cells, providing an unlimited cell resource, with potential for studying disease and use in regenerative medicine. However, genetic manipulation and technically challenging strategies such as nuclear transfer used in reprogramming limit their clinical applications. Here, we show that pluripotent stem cells can be generated from mouse somatic cells at a frequency up to 0.2% using a combination of seven small-molecule compounds. The chemically induced pluripotent stem cells resemble embryonic stem cells in terms of their gene expression profiles, epigenetic status, and potential for differentiation and germline transmission. By using small molecules, exogenous "master genes" are dispensable for cell fate reprogramming. This chemical reprogramming strategy has potential use in generating functional desirable cell types for clinical applications. |
| URI | http://hdl.handle.net/20.500.11897/319928 |
| ISSN | 0036-8075 |
| DOI | 10.1126/science.1239278 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 生命科学学院 化学生物学与生物技术学院 |
