| Title | Biofilm formation of salivary microbiota on dental restorative materials analyzed by denaturing gradient gel electrophoresis and sequencing |
| Authors | Wang, Shuai Guo, Lihong Seneviratne, Chaminda Jayampath Huang, Bo Han, Jianmin Peng, Lei Liu, Xiaodi Zhang, Chengfei |
| Affiliation | Univ Hong Kong, Fac Dent, Sai Ying Pun, Hong Kong, Peoples R China. Peking Univ, Sch & Hosp Stomatol, Dept Oral Biol, Beijing 100081, Peoples R China. Peking Univ, Sch & Hosp Stomatol, Natl Engn Lab Digital & Mat Technol Stomatol, Dent Mat Lab, Beijing 100081, Peoples R China. Univ Calif Los Angeles, Sch Dent, Los Angeles, CA 90095 USA. Univ Hong Kong, Fac Dent, 34 Hosp Rd, Sai Ying Pun, Hong Kong, Peoples R China. |
| Keywords | Dental restorative materials Biofilm Denaturing gradient gel electrophoresis Microbial diversity GLASS-IONOMER CEMENTS STREPTOCOCCUS-MUTANS COMMUNITIES IDENTIFICATION FLUORIDE PLAQUE POPULATIONS COMPOSITE AMALGAM GENES |
| Issue Date | 2014 |
| Publisher | dental materials journal |
| Citation | DENTAL MATERIALS JOURNAL.2014,33,(3),325-331. |
| Abstract | The microbial diversity of biofilms formed on the surfaces of amalgam, glass-ionomer cement, and resin composite was analyzed by denaturing gradient gel electrophoresis (DGGE). The V2-V3 region of salivary microbial 16S rDNA gene sequences of planktonic and biofilm bacteria, after 1 day and 1 week of incubation, was amplified by polymerase chain reaction (PCR) and analyzed by DGGE. The amounts of strongly adherent phylotypes after 1 day and 1 week on the three dental restorative materials were more than those on hydroxyapatite. Streptococcus salivarius was detected in both loosely adherent and strong adherent groups of all 1-day samples. At 1 week, the amounts of loosely adherent and strongly adherent phylotypes present on the three restorative materials ranked in this ascending order: glass-ionomer cement < resin composite < amalgam. Results of DGGE analysis suggested that glass-ionomer cement was the best material of choice in terms of suppressing bacterial phylotypes in biofilms. |
| URI | http://hdl.handle.net/20.500.11897/318703 |
| ISSN | 0287-4547 |
| DOI | 10.4012/dmj.2013-152 |
| Indexed | SCI(E) EI |
| Appears in Collections: | 口腔医院 |
