| Title | Nimotuzumab suppresses epithelial-mesenchymal transition and enhances apoptosis in low-dose UV-C treated salivary adenoid cystic carcinoma cell lines in vitro |
| Authors | Jiang, Yang Ge, Xi-Yuan Liu, Shu-Ming Zheng, Lei Huang, Ming-Wei Shi, Yan Fu, Jia Zhang, Jian-Guo Li, Sheng-Lin |
| Affiliation | Peking Univ, Sch & Hosp Stomatol, Dept Oral & Maxillofacial Surg, Beijing 100081, Peoples R China. Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100081, Peoples R China. Peking Univ, Sch & Hosp Stomatol, Dept Oral & Maxillofacial Surg, 22 South Zhongguancun Ave, Beijing 100081, Peoples R China. |
| Keywords | epidermal growth factor receptor epithelial-mesenchymal transition Nimotuzumab salivary adenoid cystic carcinoma ultraviolet-C EPIDERMAL-GROWTH-FACTOR PANCREATIC-CANCER CELLS HUMAN IMMUNE-SYSTEM FACTOR RECEPTOR ULTRAVIOLET-RADIATION MOLECULAR-MECHANISMS EGFR METASTASIS ANTIBODY IRRADIATION |
| Issue Date | 2014 |
| Publisher | anti cancer drugs |
| Citation | ANTI-CANCER DRUGS.2014,25,(9),1052-1060. |
| Abstract | Salivary adenoid cystic carcinoma (SACC), which is one of the most common malignant tumors of the salivary glands, is associated with a poor long-term outcome. There are currently few therapeutic options for patients with SACC. Recent studies have shown the potential of the application of ultraviolet-C (UV-C) irradiation for the treatment of human cancer. In the present study, we investigated the effects of UV-C in the SACC cell lines SACC-83 and SACC-LM. High-dose UV-C (200 J/m(2)) induced apoptosis and inhibited colony formation significantly. However, low-dose UV-C (10 J/m(2)), which had little effect on apoptosis and colony formation, increased the ability of migration in SACC cells accompanied by a decrease in E-cadherin and an increase in vimentin, suggesting the occurrence of epithelial-mesenchymal transition (EMT). Low-dose UV-C (10 J/m(2)) also resulted in upregulation of the phosphorylated forms of epidermal growth factor receptor (EGFR) and Akt (p-EGFR and p-Akt, respectively). Pretreatment with Nimotuzumab, an anti-EGFR monoclonal antibody, reversed the EMT as well as upregulation of p-EGFR/p-Akt induced by UV-C. Moreover, Nimotuzumab enhanced UV-C induced apoptosis and inhibition of colony formation. Our results indicate that EMT exerts a protective effect against apoptosis induced by low-dose UV-C. Thus, the combined application of Nimotuzumab and low-dose UV-C in vitro has an advantageous antitumor effect in SACC compared with the application of UVC alone. Anti-Cancer Drugs 25:1052-1060 (C) 2014 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins. |
| URI | http://hdl.handle.net/20.500.11897/318345 |
| ISSN | 0959-4973 |
| DOI | 10.1097/CAD.0000000000000139 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 口腔医院 |
