| Title | Microfluidic single-cell whole-transcriptome sequencing |
| Authors | Streets, Aaron M. Zhang, Xiannian Cao, Chen Pang, Yuhong Wu, Xinglong Xiong, Liang Yang, Lu Fu, Yusi Zhao, Liang Tang, Fuchou Huang, Yanyi |
| Affiliation | Peking Univ, Biodynam Opt Imaging Ctr BIOPIC, Beijing 100871, Peoples R China. Peking Univ, Coll Engn, Beijing 100871, Peoples R China. Peking Univ, Sch Life Sci, Beijing 100871, Peoples R China. Univ Sci & Technol Beijing, Res Ctr Bioengn & Sensing Technol, Beijing 100083, Peoples R China. |
| Keywords | genomics lab on chip embryonic stem cell EMBRYONIC STEM-CELLS MESSENGER-RNA-SEQ GENE-EXPRESSION HETEROGENEITY AMPLIFICATION STOCHASTICITY SMART-SEQ2 LANDSCAPE LEVEL QPCR |
| Issue Date | 2014 |
| Publisher | proceedings of the national academy of sciences of the united states of america |
| Citation | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA.2014,111,(19),7048-7053. |
| Abstract | Single-cell whole-transcriptome analysis is a powerful tool for quantifying gene expression heterogeneity in populations of cells. Many techniques have, thus, been recently developed to perform transcriptome sequencing (RNA-Seq) on individual cells. To probe subtle biological variation between samples with limiting amounts of RNA, more precise and sensitive methods are still required. We adapted a previously developed strategy for single-cell RNA-Seq that has shown promise for superior sensitivity and implemented the chemistry in a microfluidic platform for single-cell whole-transcriptome analysis. In this approach, single cells are captured and lysed in a microfluidic device, where mRNAs with poly(A) tails are reverse-transcribed into cDNA. Double-stranded cDNA is then collected and sequenced using a next generation sequencing platform. We prepared 94 libraries consisting of single mouse embryonic cells and technical replicates of extracted RNA and thoroughly characterized the performance of this technology. Microfluidic implementation increased mRNA detection sensitivity as well as improved measurement precision compared with tube-based protocols. With 0.2 M reads per cell, we were able to reconstruct a majority of the bulk transcriptome with 10 single cells. We also quantified variation between and within different types of mouse embryonic cells and found that enhanced measurement precision, detection sensitivity, and experimental throughput aided the distinction between biological variability and technical noise. With this work, we validated the advantages of an early approach to single-cell RNA-Seq and showed that the benefits of combining microfluidic technology with high-throughput sequencing will be valuable for large-scale efforts in single-cell transcriptome analysis. |
| URI | http://hdl.handle.net/20.500.11897/315637 |
| ISSN | 0027-8424 |
| DOI | 10.1073/pnas.1402030111 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 生物医学前沿创新中心 工学院 生命科学学院 |
