| Title | Proteome reference map and regulation network of neonatal rat cardiomyocyte |
| Authors | Li, Zi-jian Liu, Ning Han, Qi-de Zhang, You-yi |
| Affiliation | Peking Univ, Hosp 3, Inst Vasc Med, Beijing 100191, Peoples R China. Minist Hlth, Key Lab Cardiovasc Mol Biol & Regulatory Peptides, Beijing 100191, Peoples R China. Minist Educ, Key Lab Mol Cardiol, Beijing 100191, Peoples R China. Jilin Univ, Hosp 2, Cent Lab, Changchun 130041, Peoples R China. |
| Keywords | proteome neonatal rat cardiomyocyte regulation network 2-DE database 2-DIMENSIONAL ELECTROPHORESIS DATABASE HEART PROTEINS IDENTIFICATION CONSTRUCTION HSC-2DPAGE MUSCLE DOG |
| Issue Date | 2011 |
| Publisher | 中国药理学报 |
| Citation | ACTA PHARMACOLOGICA SINICA.2011,32,(9),1116-1127. |
| Abstract | Aim: To study and establish a proteome reference map and regulation network of neonatal rat cardiomyocyte. Methods: Cultured cardiomyocytes of neonatal rats were used. All proteins expressed in the cardiomyocytes were separated and identified by two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). Biological networks and pathways of the neonatal rat cardiomyocytes were analyzed using the Ingenuity Pathway Analysis (IPA) program (www.ingenuity.com). A 2-DE database was made accessible on-line by Make2ddb package on a web server. Results: More than 1000 proteins were separated on 2D gels, and 148 proteins were identified. The identified proteins were used for the construction of an extensible markup language-based database. Biological networks and pathways were constructed to analyze the functions associate with cardiomyocyte proteins in the database. The 2-DE database of rat cardiomyocyte proteins can be accessed at http://2d.bjmu.edu.cn. Conclusion: A proteome reference map and regulation network of the neonatal rat cardiomyocytes have been established, which may serve as an international platform for storage, analysis and visualization of cardiomyocyte proteomic data. |
| URI | http://hdl.handle.net/20.500.11897/309449 |
| ISSN | 1671-4083 |
| DOI | 10.1038/aps.2011.86 |
| Indexed | SCI(E) PubMed 中国科技核心期刊(ISTIC) 中国科学引文数据库(CSCD) |
| Appears in Collections: | 第三医院 |
