| Title | An Outbreak of a Nosocomial NDM-1-Producing Klebsiella pneumoniae ST147 at a Teaching Hospital in Mainland China |
| Authors | Wang, Xiaojuan Xu, Xiuli Li, Zongwei Chen, Hongbin Wang, Qi Yang, Peihong Zhao, Chunjiang Ni, Ming Wang, Hui |
| Affiliation | Peking Univ, Peoples Hosp, Dept Clin Lab, Beijing 100044, Peoples R China. Fourth Mil Med Univ, Xijing Hosp, Dept Clin Lab, Xian 710032, Peoples R China. Beijing Inst Radiat Med, Dept Bioinformat, Beijing, Peoples R China. |
| Keywords | CARBAPENEM-RESISTANT ENTEROBACTERIACEAE BETA-LACTAMASES BLA(NDM-1) SEQUENCE BAUMANNII EMERGENCE GENE |
| Issue Date | 2014 |
| Publisher | microbial drug resistance |
| Citation | MICROBIAL DRUG RESISTANCE.2014,20,(2),144-149. |
| Abstract | A total of 1,870 nonduplicate clinical Enterobacteriaceae from 13 teaching hospitals located in 11 provinces of mainland China from 2011 to 2012 were screened for the presence of the bla(NDM) gene. The high-throughput MiSeq sequencing method and comparative genomics were used to analyze the genetic environment of bla(NDM) among these isolates. Three bla(NDM-1)-carrying Klebsiella pneumoniae (0.16%, 3/1,870), isolated from a teaching hospital in Xi'an, exhibited high levels of resistance to all -lactams, but remained susceptible to amikacin, tigecycline, and polymyxin B. These three isolates, belonging to ST147, presented an identical pulsed-field gel electrophoresis pattern. The IncX3 plasmid, pNDM-SX04 (KC876051) showed 99% identity with plasmid pNDM-HN380 (JX104760). Comparative analysis of the genetic environment of bla(NDM-1) with previously published plasmids revealed the same 7,830-bp basic mobile element, which may have been derived from Acinetobacter spp. Partial ISAba125, ISAba125 promoter, bla(NDM-1), and ble(MBL) could serve as the minimal mobile vehicle facilitating horizontal transfer of the bla(NDM-1) gene. To our knowledge, this is the first report of an outbreak of bla(NDM-1)-carrying ST147 K. pneumoniae. Although the prevalence spread by the bla(NDM-1) gene prevalence is at a low frequency in mainland China, a dynamic national surveillance of this gene is needed due to its potential transferability. |
| URI | http://hdl.handle.net/20.500.11897/307053 |
| ISSN | 1076-6294 |
| DOI | 10.1089/mdr.2013.0100 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 人民医院 |
