| Title | LncRNA specific for distant metastasis of gastric cancer is associated with TRIM16 expression and facilitates tumor cell invasion in vitro |
| Authors | Yan Yichao Shen Zhanlong Gao Zhidong Cao Jian Yang Yang Wang Bo Master Chao Shen Mao Shuqiang Jiang Kewei Ye Yingjiang Wang Shan |
| Affiliation | Department of Gastroenterological Surgery, Department of Surgical Oncology, Peking University People's Hospital, Beijing 100044, PR China. |
| Keywords | Gastric cancer,Invasion,LOC100507069,Long non-coding RNA,Metastasis |
| Issue Date | 2015 |
| Publisher | journal of gastroenterology and hepatology |
| Citation | Journal of gastroenterology and hepatology.2015. |
| Abstract | Increasing evidence has indicated that long non-coding RNAs (lncRNAs) play a major role in cancers. Although certain lncRNAs has been reported to play a role in gastric cancer (GC), specific lncRNAs involved in distant metastasis of GC remain unknown.Differentially expressed mRNAs and lncRNAs between stage IV and non-stage IV GC were obtained by microarray. Gene Ontology (GO) and pathway analysis were used to study functions of differential mRNAs. Algorithms were used to predict potential gene targets of cis or trans-acting lncRNAs. Network analysis was performed to analyze each pair of gene-lncRNA, gene-gene or lncRNA-lncRNA interactions. Expression of lncRNA special for distant metastasis of GC (SDMGC) and target gene TRIM16 were tested in GC tissues and cell lines. RNAi and over-expression were used to observe the biological functions of SDMGC and TRIM16 on GC cells.502 mRNAs and 440 lncRNAs were found to be differentially expressed. 74 GO terms and 38 pathways were associated with the dysregulated transcripts. Fourteen core factors were determined by network analysis. Expression of SDMGC and TRIM16 were up-regulated in the distant metastasis tissues, compared to primary GC tissues, which were positive correlation. Silencing of SDMGC or TRIM16 was demonstrated to decrease cell invasion and migration, while up-regulated of SDMGC or TRIM16 could promote cell invasion and migration. However, little effect on proliferation, cell cycle, colony formation and apoptosis were found.SDMGC is obviously up-regulated in stage IV GC and may represent a new marker and therapeutic target for GC treatment.This article is protected by copyright. All rights reserved. |
| URI | http://hdl.handle.net/20.500.11897/306859 |
| ISSN | 1440-1746 |
| DOI | 10.1111/jgh.12976 |
| Indexed | SCI(E) PubMed |
| Appears in Collections: | 人民医院 |
