| Title | Biodegradable Poly(lactic-co-glycolic acid) Maxpol-T/S as Novel Scaffold for Adipose Derived Stem Cells and Fibroblast Growth In Vitro |
| Authors | Jin, Zhe Gong, Yan-Qing Qin, Xin-Cheng Zhang, Jian Zhu, Yi-Chen Lin, Gui-ting Lue, Tom F. Xin, Zhong-Cheng |
| Affiliation | Peking Univ, Androl Ctr, Hosp 1, Beijing 100034, Peoples R China. Univ Calif San Francisco, Dept Urol, Sch Med, San Francisco, CA 94143 USA. |
| Keywords | Adipose-derived stem cells fibroblasts GFP PLGA mesh cell adhesion tissue engineering VENOUS PATCH GRAFT PEYRONIES-DISEASE DIFFERENTIATION REGENERATION |
| Issue Date | 2008 |
| Publisher | tissue engineering and regenerative medicine |
| Citation | TISSUE ENGINEERING AND REGENERATIVE MEDICINE.2008,5,(4-6),869-876. |
| Abstract | To explore the ability of MaxPol-T/S, a novelbiodegradable poly-lactic-co-glycolic acid(PLGA) scaffold, in providing a cell-matrix interaction interface for cell growth, we generated GFP positive adipose derived stem cell(ADSC(GFP+)) and fibroblasts(FC(GFP+)) for investigating the cellular growth on this biomaterial. The MaxPol-T/S was produced through salt-leaching/particulate-leaching technology for tissue engineering, which provides a modified surface for the best cellular attachment sites and nutrient supply and waste exchange conditions. The morphological features of MaxPol-T/S were studied with a scanning electron microscope and the ADSC(GFP+) and FC(GFP+) were confirmed by auto-fluorescence microscopy and flow cytometry assay. Being seeded onto the MaxPol-T/S in vitro, the growth and morphology of ADSC(GFP+) and FC(GFP+) were further verified by auto-fluorescence microscopy and MMT test. The ADSCs and fibroblasts, expressed strong GFP signals in the cytoplasm and nucleus, adhered and proliferated on the surface of scaffold MaxPol-T/S. Both cell lines survived on the scaffold more than 21 days in vitro and formed three-dimensional colonies on the surface of the MaxPol-T. In conclusion, MaxPol-T/S is a novel PLGA scaffold for ADSC(GFP+) and FC(GFP+) and implies a promising technique for tissue engineering. |
| URI | http://hdl.handle.net/20.500.11897/305915 |
| ISSN | 1738-2696 |
| Indexed | SCI(E) EI |
| Appears in Collections: | 第一医院 |
