Title | Celecoxib inhibits Helicobacter pylori colonization-related factors |
Authors | Wang, Jing Wang, Wei-Hong Li, Jiang Liu, Fang-Xun |
Affiliation | Peking Univ, Hosp 1, Dept Gastroenterol, Beijing 100034, Peoples R China. Peking Univ, Hosp 1, Dept Gastroenterol, 8 Xishiku Ave, Beijing 100034, Peoples R China. |
Keywords | Helicobacter pylori Celecoxib Colonization Ultrastructure NONSTEROIDAL ANTIINFLAMMATORY DRUGS UREASE ACTIVITY PEPTIC-ULCERATION GASTRIC-CANCER IN-VIVO INFECTION MOTILITY ADHESIN NSAIDS RISK |
Issue Date | 2010 |
Publisher | world journal of gastroenterology |
Citation | WORLD JOURNAL OF GASTROENTEROLOGY.2010,16,(7),846-853. |
Abstract | AIM: To investigate the effect of celecoxib, a selective COX-2 inhibitor, on Helicobacter pylori (H. pylori) colonization-related factors and its mechanism. METHODS: After co-incubation with celecoxib, morphology of H. pylori strain 26695 was observed under a transmission electron microscope. Flagella motility was assessed by stab agar motility test. Adherence of H. pylori to AGS cells was determined by enzyme linked immunosorbent assay. Levels of mRNA expression in flagellar genes (flaA, flaB), urease genes (ureA, ureB) and adhesin genes (babA, sabA, alpA, alpB, hpaA, hopZ) were measured by real-time polymerase chain reaction. RESULTS: Separation and non-integrity of bacterial cell wall, rarefaction and asymmetry of cytoplasm, and even lysis of H. pylori were observed in the presence of celecoxib. When H. pylori strains were incubated in the presence of celecoxib, their flagellar motility and adherence to AGS cells were inhibited. The expression Of ureA, ureB, babA, sabA, alpA, alpB, hpaA, hopZ was up-regulated while the expression of flaA, flaB was down-regulated in the presence of celecoxib. CONCLUSION: Celecoxib inhibits flagellar motility and adherence of H. pylori to AGS cells, and destructs their normal structure in vitro. (C) 2010 Baishideng. All rights reserved. |
URI | http://hdl.handle.net/20.500.11897/305857 |
ISSN | 1007-9327 |
DOI | 10.3748/wjg.v16.i7.846 |
Indexed | SCI(E) 中国科技核心期刊(ISTIC) 中国科学引文数据库(CSCD) |
Appears in Collections: | 第一医院 |