Title | Structures and immunological activities of two pectic polysaccharides from the fruits of Ziziphus jujuba Mill. cv. jinsixiaozao Hort. |
Authors | Zhao, Zhihui Li, Jun Wu, Xiangmei Dai, Hui Gao, Xiaoming Liu, Mengjun Tu, Pengfei |
Affiliation | Agri Univ Hebei, Res Ctr Chinese Jujube, Hebei 071001, Peoples R China. Peking Univ, Hlth Sci Ctr, Modern Res Ctr Tradit Chinese Med, Sch Pharmaceut Sci, Beijing 100083, Peoples R China. Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Beijing 100083, Peoples R China. |
Keywords | Ziziphus jujuba Mill. cv. jinsixiaozao Hort. fruit pectic polysaccharide immunological activity ZIZYPHUS-JUJUBA METHYLATION DEGRADATION SUBSTANCES |
Issue Date | 2006 |
Publisher | food research international |
Citation | FOOD RESEARCH INTERNATIONAL.2006,39,(8),917-923. |
Abstract | Two pectic polysaccharides were isolated from Ziphus jujuba Mill. cv. jinsixiaozao Hort. and their structures were characterized by acid hydrolysis, methylation, partial acid hydrolysis and NMR spectroscopy. The purified fraction Ju-B-3 had a molecular mass of over 2000 kDa and showed [alpha](D)(20) + 151 (c = 1.00, H2O). Chemical and spectroscopic analyses indicated that Ju-B-3 was a polygalacturonan D with 7.49% methoxylation. The purified fraction Ju-B-2 had a molecular weight of over 2000 kDa and showed [alpha](D)(20) + 125 (c = 1.00, D H2O). Ju-B-2 was proposed as a polygalacturonan interspersed with rhamnogalacturonan in the main chain. 10.47% D-galacturonic acid existed as methoxylation and O-acetyl groups were amounted to 2.5%. The branched side chains were mainly composed of 1,5-linked arabinofuranosyl residues and 1,6-linked galactopyranose residues attached to O-4 of rhamnose residues. Ju-B-2 induced a rat spleen cells proliferation in a dose-dependent manner while Ju-B-3 could not show any proliferation activity compared with control. (c) 2006 Elsevier Ltd. All rights reserved. |
URI | http://hdl.handle.net/20.500.11897/252907 |
ISSN | 0963-9969 |
DOI | 10.1016/j.foodres.2006.05.006 |
Indexed | SCI(E) EI |
Appears in Collections: | 基础医学院 |