| Title | Isolation and identification of an scFv antibody against nucleocapsid protein of SARS-CoV |
| Authors | Zhao, Aizhi Qin, Weijun Han, Yueheng Wen, Weihong Zhang, Wenhong Lian, Zhonghui Chen, Gang Zhang, Zhuoli Peng, Jianqiang Wang, He Guo, Yinglu |
| Affiliation | Vector Gene Technol Co Ltd, Beijing 100176, Peoples R China. Fourth Mil Med Univ, Xijing Hosp, Dept Urol, Xian 710032, Peoples R China. Fourth Mil Med Univ, Dept Biochem & Biol, Xian 710032, Peoples R China. Peking Univ, Hosp 1, Inst Urol, Beijing 100034, Peoples R China. |
| Keywords | SARS-CoV scFv nucleocapsid phage display RESPIRATORY SYNDROME SARS LINKED-IMMUNOSORBENT-ASSAY POLYMERASE-CHAIN-REACTION ESCHERICHIA-COLI MONOCLONAL-ANTIBODIES SYNDROME CORONAVIRUS FRAGMENTS PNEUMONIA INFECTION |
| Issue Date | 2007 |
| Publisher | microbes and infection |
| Citation | MICROBES AND INFECTION.2007,9,(8),1026-1033. |
| Abstract | To develop reagents for early diagnosis and therapeutic drugs against SARS-associated coronavirus (SARS-CoV), a large (3 x 109) immunized human antibody library was constructed from peripheral blood mononuclear cells from six SARS convalescent patients. A single chain variable fragment antibody (N18) with high affinity against N protein of SARS-CoV was isolated. Sequence analysis revealed that the VL gene was composed of VL3 h (V lambda subgroup) and JL2 regions and the VH gene was composed of VH1-69 (VH I subgroup), D2-15, D3-22 and JH6 regions. Soluble N18 antibody was expressed in Escherichia coli HB2151, purified by Ni-NTA affinity chromatography and verified by SDS-PAGE and Western blot. The potential application for early diagnosis was evaluated using N protein capture ELISA in which N 18 antibody demonstrated high sensitive activity in detecting N protein of SARS-CoV. Finally, the potential usefulness of the N18 antibody in prophylaxis, vaccine design and therapy of SARS is discussed. (c) 2007 Elsevier Masson SAS. All rights reserved. |
| URI | http://hdl.handle.net/20.500.11897/250224 |
| ISSN | 1286-4579 |
| DOI | 10.1016/j.micinf.2007.04.008 |
| Indexed | SCI(E) |
| Appears in Collections: | 第一医院 |
